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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
Tazobactam Impurity 14 Tazobactam Impurity 14
Ticagrelor Impurity 124 Ticagrelor Impurity 124
Tofacitinib Impurity 113 Tofacitinib Impurity 113
Voriconazole Impurity 20 Voriconazole Impurity 20
Carbocistein Impurity F Carbocistein Impurity F
Cilastatin EP Impurity B Cilastatin EP Impurity B
Olaparib Impurity 14 Olaparib Impurity 14 2250242-62-5 C36H28F2N6O4
Brimonidine Impurity B Brimonidine Impurity B
Diquafosol Impurity C Diquafosol Impurity C
Bortezomib Impurity 19 Bortezomib Impurity 19
Apixaban Impurity 40 Apixaban Impurity 40
Axitinib Impurity 3 Axitinib Impurity 3
Sofosbuvir Impurity 10 Sofosbuvir Impurity 10
Venlafaxine Impurity 10 Venlafaxine Impurity 10 1997279-63-6 C17H27NO2
Dapoxetine Impurity 3 Dapoxetine Impurity 3
Regorafenib Impurity 16 Regorafenib Impurity 16
Topiroxostat Impurity 7 Topiroxostat Impurity 7
Tofacitinib Impurity 12 Tofacitinib Impurity 12
Mirabegron iMpurity Mirabegron iMpurity 1581285-26-8 C16H21ClN2
Sunitinib iMpurity Sunitinib iMpurity 356068-98-9 C12H19N3O2
(-)-DihydroapovincaMinic Acid Ethyl Ester (-)-DihydroapovincaMinic Acid Ethyl Ester 57327-92-1 C22H28N2O2
(α1S,α4S,2S,5S)-2,5-DiMethyl-3,6-dioxo-α1,α4-bis(2-phenylethyl)-1,4-piperazinediacetic Acid 1,4-Diethyl Ester (α1S,α4S,2S,5S)-2,5-DiMethyl-3,6-dioxo-α1,α4-bis(2-phenylethyl)-1,4-piperazinediacetic Acid 1,4-Diethyl Ester 151387-05-2 C30H38N2O6
[2-(4-Fluorophenyl)cyclopropyl]aMine hydrochloride [2-(4-Fluorophenyl)cyclopropyl]aMine hydrochloride 879324-66-0 C9H11ClFN
[4-(4-AMino-7-hydroxy-6-Methoxy-2-quinazolinyl)-1-piperazinyl](tetrahydro-2-furanyl)Methanone [4-(4-AMino-7-hydroxy-6-Methoxy-2-quinazolinyl)-1-piperazinyl](tetrahydro-2-furanyl)Methanone 105356-90-9 C18H23N5O4
2-(DiethylaMino)-N-(2,5-diMethylphenyl)acetaMide Hydrochloride 2-(DiethylaMino)-N-(2,5-diMethylphenyl)acetaMide Hydrochloride 1012864-23-1 C14H23ClN2O
2-Keto-D-gulonic Acid Methyl Ester 2-Keto-D-gulonic Acid Methyl Ester 67776-07-2 C7H12O7
9,11-Dehydro-17α-cyanoMethyl Estradiol 9,11-Dehydro-17α-cyanoMethyl Estradiol 86153-38-0 C20H23NO2
DeMethyl Irbesartan DeMethyl Irbesartan 158778-58-6 C24H26N6O
Epiprednisolone Epiprednisolone 600-90-8 C21H28O5
δ-2-CefodiziMe (2 :1 Mixture) δ-2-CefodiziMe (2 :1 Mixture) 120533-30-4 C20H20N6O7S4
Salbutamol Acetonide Salbutamol Acetonide 54208-72-9 C16H25NO3
8-Chloro-1-methyl-6-phenyl-4H-Imidazo[1,5-a][1,4]benzodiazepine 8-Chloro-1-methyl-6-phenyl-4H-Imidazo[1,5-a][1,4]benzodiazepine 59467-86-6 C18H14ClN3
BenzeneMethanaMine, 4-ethoxy-3-Methoxy-a-[(Methylsulfonyl)Methyl]- BenzeneMethanaMine, 4-ethoxy-3-Methoxy-a-[(Methylsulfonyl)Methyl]- 885268-06-4 C12H19NO4S
Afatinib IMpurity C Afatinib IMpurity C
Afatinib IMpurity C Afatinib IMpurity C
AMisulpride IMpurity AMisulpride IMpurity 84859-26-7 C8H17NO2
Avanafil iMpurity 5 Avanafil iMpurity 5
Cefoxitin iMpurity E (R-Methoxy cefoxitin) Cefoxitin iMpurity E (R-Methoxy cefoxitin)
Desloratadine IMpurity 3 Desloratadine IMpurity 3 1346600-61-0 C19H19ClN2
DibroMo IMpurity DibroMo IMpurity 1352492-08-0 C15H12Br2O2
Ticagrelor Impurity 16 Ticagrelor Impurity 16
Ticagrelor Impurity 22 Ticagrelor Impurity 22
Milrinone Impurity 5 Milrinone Impurity 5
Caspofungin Impurity 4 Caspofungin Impurity 4 258510-40-6 C49H78N8O17
Canagliflozin Impurity 27 Canagliflozin Impurity 27
Loxoprofen Impurity 1 Loxoprofen Impurity 1
Febuxostat Impurity 54 Febuxostat Impurity 54
Silodosin Impurity B Silodosin Impurity B
Cefminox Sodium impurity F Cefminox Sodium impurity F
Clindamycin Impurity F Clindamycin Impurity F
Fasudil iMpurity D Fasudil iMpurity D
2-((4R,6R)-6-(2-(2-(4-fluorophenyl)-5-isopropyl-3-phenyl-4-(phenylcarbamoyl)-1H-pyrrol-1-yl)ethyl)-2,2-dimethyl-1,3-dioxan-4-yl)acetic acid 2-((4R,6R)-6-(2-(2-(4-fluorophenyl)-5-isopropyl-3-phenyl-4-(phenylcarbamoyl)-1H-pyrrol-1-yl)ethyl)-2,2-dimethyl-1,3-dioxan-4-yl)acetic acid 929540-41-0 C36H41CaFN2O5
Doxofylline iMpurity C Doxofylline iMpurity C
Puerarin-4'-O-β-D-glucopyranoside Puerarin-4'-O-β-D-glucopyranoside 117047-08-2 C27H30O14
parecoxib impurity L parecoxib impurity L
Rupatadine iMpurity Rupatadine iMpurity 169253-15-0 C19H19ClN2
ceftazidiMe iMpurity C ceftazidiMe iMpurity C
pidotiMod iMpurity E pidotiMod iMpurity E 162148-16-5 C9H12N2O4S
1H-Pyrazole-1-propanenitrile, β-cyclopentyl-4-[7-(hydroxyMethyl)-7H-pyrrolo[2,3-d]pyriMidin-4-yl]-,(βR)- 1H-Pyrazole-1-propanenitrile, β-cyclopentyl-4-[7-(hydroxyMethyl)-7H-pyrrolo[2,3-d]pyriMidin-4-yl]-,(βR)- 1236033-03-6 C18H20N6O
Cyclopropanecarboxylic acid, 1-[(phenylaMino)carbonyl]- Cyclopropanecarboxylic acid, 1-[(phenylaMino)carbonyl]- 145591-80-6 C11H11NO3
3-PiperidinaMine, 1-acetyl-N,4-diMethyl-N-1H-pyrrolo[2,3-d]pyriMidin-4-yl-, (3R,4R)- (9CI) 3-PiperidinaMine, 1-acetyl-N,4-diMethyl-N-1H-pyrrolo[2,3-d]pyriMidin-4-yl-, (3R,4R)- (9CI) 477600-76-3 C15H21N5O
AzithroMycin iMpurity AzithroMycin iMpurity 194809-66-0 C37H69BN2O13
MeropeneM iMpurity MeropeneM iMpurity 96035-23-3 C15H19N3O5S
Ciprofloxacin EP IMpurity B Ciprofloxacin EP IMpurity B 93107-11-0 C17H19N3O3
Valdecoxib IMpurity-E Valdecoxib IMpurity-E 1373038-63-1 C16H11Cl2NO5S2
Sunitinib N-Oxide Sunitinib N-Oxide 356068-99-0 C22H27FN4O3
ErtapeneM IMpurity DIPP ErtapeneM IMpurity DIPP 220031-86-7 C18H27N2O6PS
Gefitinib IMpurity 1 Gefitinib IMpurity 1 1561173-24-7 C15H22ClN3O3
Pitavastatin Defuoro IMpurity CalciuM Salt Pitavastatin Defuoro IMpurity CalciuM Salt 1258947-30-6 C50H50CaN2O8
Solifenacin Related CoMpound 4 Succinate Solifenacin Related CoMpound 4 Succinate 862207-71-4 C27H32N2O6
TenoxicaM IMpurity E (2-Methylthieno[2,3-d]isothiazol-3(2H)-one-1,1-dioxide ) TenoxicaM IMpurity E (2-Methylthieno[2,3-d]isothiazol-3(2H)-one-1,1-dioxide ) 94040-09-2 C6H5NO3S2
(+)-(14β)-Dihydrovinpocetine (+)-(14β)-Dihydrovinpocetine 57517-54-1 C22H28N2O2
D,L-Venlafaxine-d10 Hydrochloride D,L-Venlafaxine-d10 Hydrochloride 1216539-56-8 C17H28ClNO2
Ceritinib IMpurity Ceritinib IMpurity
Melphalan IMpurity Melphalan IMpurity
1-[2-[(5-chloro-2,4-diMethylphenyl)thio]phenyl]- Piperazine 1-[2-[(5-chloro-2,4-diMethylphenyl)thio]phenyl]- Piperazine 1240670-87-4 C18H21ClN2S
4-(4-(4-(4-(((3S,5S)-5-((1H-1,2,4-triazol-1-yl)Methyl)-5-(2,4-difluorophenyl)tetrahydrofuran-3-yl)Methoxy)phenyl)piperazin-1-yl)phenyl)-1-((2R,3R)-2-hydroxypentan-3-yl)-1H-1,2,4-triazol-5(4H)-one 4-(4-(4-(4-(((3S,5S)-5-((1H-1,2,4-triazol-1-yl)Methyl)-5-(2,4-difluorophenyl)tetrahydrofuran-3-yl)Methoxy)phenyl)piperazin-1-yl)phenyl)-1-((2R,3R)-2-hydroxypentan-3-yl)-1H-1,2,4-triazol-5(4H)-one 2243785-97-7 C37H42F2N8O4
4-(4-broMo-3-(hydroxyMethyl)phenoxy)benzonitrile 4-(4-broMo-3-(hydroxyMethyl)phenoxy)benzonitrile 906673-45-8 C14H10BrNO2
ACT 080803 ACT 080803 1433875-21-8 C16H13Br2N5O2
L-Alanine, N-[(S)-hydroxyphenoxyphosphinyl]-, 1-Methylethyl ester, (P→3'),(P'→5')-diester with (2'R)-2'-deoxy-2'-fluoro-2'-Methyluridine L-Alanine, N-[(S)-hydroxyphenoxyphosphinyl]-, 1-Methylethyl ester, (P→3'),(P'→5')-diester with (2'R)-2'-deoxy-2'-fluoro-2'-Methyluridine 1337482-17-3 C34H45FN4O13P2
Pyrido[2,3-d]pyriMidin-7(8H)-one, 2,6-dibroMo-8-cyclopentyl-5-Methyl- Pyrido[2,3-d]pyriMidin-7(8H)-one, 2,6-dibroMo-8-cyclopentyl-5-Methyl- 1415560-29-0 C13H13Br2N3O
1,1'-[1,3-Propanediylbis(oxy)]bis[2,5-dimethylbenzene] 1,1'-[1,3-Propanediylbis(oxy)]bis[2,5-dimethylbenzene] 415724-02-6 C19H24O2
1-Dimethylamino-2-phenylbutan-2-yl 3,4,5-trimethoxybenzoate 1-Dimethylamino-2-phenylbutan-2-yl 3,4,5-trimethoxybenzoate 92414-09-0 C22H29NO5
Celecoxib 2-Methyl Analog Celecoxib 2-Methyl Analog 170569-99-0 C17H14F3N3O2S
EMpagliflozin  iMpurity 6 EMpagliflozin iMpurity 6 1620758-21-5 C32H37ClO12
EMpagliflozin  iMpurity 11 EMpagliflozin iMpurity 11
1,4,7,10-Tetraazacyclododecane-1,4,7-triacetic acid,10-[2,3-dihydroxy-1-(hydroxymethyl)propyl]- 1,4,7,10-Tetraazacyclododecane-1,4,7-triacetic acid,10-[2,3-dihydroxy-1-(hydroxymethyl)propyl]- 138168-36-2 C18H34N4O9
Di-Norbudesonide (Mixture of DiastereoMers) Di-Norbudesonide (Mixture of DiastereoMers) 1040085-98-0 C23H30O6
Linagliptin MethyldiMer Linagliptin MethyldiMer 1418133-47-7 C50H56N16O4
Trelagliptin Impurity isomer Trelagliptin Impurity isomer 2206135-35-3 C18H20FN5O2
(2R,3R,4R,5S,6R)-2-(4-chloro-3-(4-ethoxybenzyl)phenyl)-6-(hydroxyMethyl)tetrahydro-2H-pyran-3,4,5-triol (2R,3R,4R,5S,6R)-2-(4-chloro-3-(4-ethoxybenzyl)phenyl)-6-(hydroxyMethyl)tetrahydro-2H-pyran-3,4,5-triol C21H25ClO6
3,6-Dichloropyrazine-2-carboxaMide 3,6-Dichloropyrazine-2-carboxaMide 1023813-21-9 C5H3Cl2N3O
N-acetic acid-1-aMino-3-adaMantanol N-acetic acid-1-aMino-3-adaMantanol 1032564-18-3 C12H19NO3
9(10H)-Acridinone, 2-ethoxy-6-nitro- 9(10H)-Acridinone, 2-ethoxy-6-nitro- 451460-48-3 C15H12N2O4
Olaparib iMpurity D Olaparib iMpurity D
(5-broMo-2-chlorophenyl)(4-hydroxyphenyl)Methanone (5-broMo-2-chlorophenyl)(4-hydroxyphenyl)Methanone 1360568-68-8 C13H8BrClO2
(2-broMo-4,5-diMethylphenyl)Methanol (2-broMo-4,5-diMethylphenyl)Methanol 1126385-20-3 C9H11BrO
Posaconazole iMpurity 8 Posaconazole iMpurity 8
LCZ Impurity LCZ Impurity 753421-85-1 C20H25NO2
carbamic acid, n-[(1r,2r,5s)-5-[(dimethylamino)carbonyl]-2-hydroxycyclohexyl]-, 1,1-dimethylethyl ester carbamic acid, n-[(1r,2r,5s)-5-[(dimethylamino)carbonyl]-2-hydroxycyclohexyl]-, 1,1-dimethylethyl ester 929693-30-1 C14H26N2O4
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