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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
Ticagrelor Impurity 71 Ticagrelor Impurity 71
Tolterodine EP Impurity G Tolterodine EP Impurity G 1554029-91-2 C22H32NO2+
Topiroxostat Impurity 23 Topiroxostat Impurity 23 2044706-78-5 C20H12N10
Saxagliptin Impurity 34 Saxagliptin Impurity 34 2173146-31-9 C17H25NO5
Afatinib Impurity 25 Afatinib Impurity 25
Bendamustine Impurity 34 Bendamustine Impurity 34
Canagliflozin Impurity 29 Canagliflozin Impurity 29
Cefazolin Impurity 6 Cefazolin Impurity 6 66636-33-7 C14H20N4O3S3Si
Ciprofloxacin Impurity 5 Ciprofloxacin Impurity 5 C17H18FN3O3
Erlotinib Impurity 53 Erlotinib Impurity 53 749181-30-4 C21H25N3O4
Etoposide Impurity 4 Etoposide Impurity 4 101648-60-6 C35H32Cl6O16
Olaparib Impurity 25 Olaparib Impurity 25 1423030-11-8 C16H9FO4
Olaparib Impurity 32 Olaparib Impurity 32
Oseltamivir Impurity 23 Oseltamivir Impurity 23 2241125-39-1 C9H16N2O3
Rosuvastatin Impurity 85 Rosuvastatin Impurity 85
4,5,6,7-Tetrahydro-thieno[3,2-c]pyridin-2-ol 2-acetate 4,5,6,7-Tetrahydro-thieno[3,2-c]pyridin-2-ol 2-acetate 1151904-85-6 C9H11NO2S
(2S,4S)-5-(Biphenyl-4-yl)-4-[(tert-butoxycarbonyl)amino]-2-methylpentanoic acid (2S,4S)-5-(Biphenyl-4-yl)-4-[(tert-butoxycarbonyl)amino]-2-methylpentanoic acid 1012341-52-4 C23H29NO4
Ethyl (2R,4S)-4-([1,1'-biphenyl]-4-ylmethyl)-2-methyl-4-(2,5-dioxopyrrolidin-1-yl)butanoate Ethyl (2R,4S)-4-([1,1'-biphenyl]-4-ylmethyl)-2-methyl-4-(2,5-dioxopyrrolidin-1-yl)butanoate 1038924-97-8 C24H27NO4
DONEPEZIL IMP DONEPEZIL IMP 923571-16-8 C24H22NO4.Cl
Parecoxib Impurity N Parecoxib Impurity N
5-[bis-(2-Chloroethyl)amino]-1-methyl-1H-benzimidazole-2-butanoic acid ethyl ester 5-[bis-(2-Chloroethyl)amino]-1-methyl-1H-benzimidazole-2-butanoic acid ethyl ester 87475-54-5 C18H25Cl2N3O2
6-Hydroxykaempferol 3-Rutinoside -6-glucoside 6-Hydroxykaempferol 3-Rutinoside -6-glucoside 145134-63-0 C33H40O21
(R)-1-(3,4-dimethoxybenzyl)-6,7-dimethoxy-2-methyl-1,2,3,4-tetrahydroisoquinoline (R)-1-(3,4-dimethoxybenzyl)-6,7-dimethoxy-2-methyl-1,2,3,4-tetrahydroisoquinoline 85-63-2 C21H27NO4
Linagliptin Impurity P Linagliptin Impurity P
Linagliptin Impurity U Linagliptin Impurity U
(R)-2-((6-(3-aminopiperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-4-fluorobenzoic acid (R)-2-((6-(3-aminopiperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-4-fluorobenzoic acid 1917324-15-2 C18H21FN4O4
N1,N4-bis((R)-1-(3-(2-cyano-5-fluorobenzyl)-1-methyl-2,6-dioxo-1,2,3,6-tetrahydropyrimidin-4-yl)piperidin-3-yl)succinamide N1,N4-bis((R)-1-(3-(2-cyano-5-fluorobenzyl)-1-methyl-2,6-dioxo-1,2,3,6-tetrahydropyrimidin-4-yl)piperidin-3-yl)succinamide C40H42F2N10O6
ethyl ((1R,3aR,4aR,6S,8aR,9S,9aS)-9-((E)-2-(5-(3-fluorophenyl)pyridin-2-yl)vinyl)-1-methyl-3-oxododecahydronaphtho[2,3-c]furan-6-yl)carbamate ethyl ((1R,3aR,4aR,6S,8aR,9S,9aS)-9-((E)-2-(5-(3-fluorophenyl)pyridin-2-yl)vinyl)-1-methyl-3-oxododecahydronaphtho[2,3-c]furan-6-yl)carbamate 618385-10-7 C29H33FN2O4
(2R)-2-deoxy-2-fluoro-2-methyl-α/β-D-erythro-pentofuranosyl chloride-3,5-dibenzoate (2R)-2-deoxy-2-fluoro-2-methyl-α/β-D-erythro-pentofuranosyl chloride-3,5-dibenzoate 1621160-31-3 C20H18ClFO5
Apremilast Impurity F Apremilast Impurity F
Etoricoxib Impurity 17 Etoricoxib Impurity 17
Ibrutinib Impurity 3 Ibrutinib Impurity 3 1226872-27-0 C25H26N6O4
Imatinib Impurity 2 Imatinib Impurity 2 1384131-91-2 C7H11NO
Mirabegron M5 (YM-538852) Mirabegron M5 (YM-538852) 1365244-63-8 C18H22N2O2
Sofosbuvir Impurity 6 Sofosbuvir Impurity 6
Vonoprazan Impurity 2 Vonoprazan Impurity 2 881733-36-4 C17H16FN3O2S
Atomoxetine EP impurity A Atomoxetine EP impurity A 56161-70-7 C16H19NO
Cinacalcet Impurity 21 Cinacalcet Impurity 21
Dasatinib Impurity D Dasatinib Impurity D
Doxofylline Impurity 1 Doxofylline Impurity 1 1429636-74-7 C10H16N4O3
Isopropyl4-hydroxy-3,5-diisopropylbenzoate Isopropyl4-hydroxy-3,5-diisopropylbenzoate 2095678-97-8 C16H24O3
Sitagliptin EP Impurity B Sitagliptin EP Impurity B 1345822-87-8 C16H19F5N5O5P
Trospium Impurity Trospium Impurity
Vildagliptin Related Compound A Vildagliptin Related Compound A 741657-02-3 C17H25N3O
2-amino-9-((1R,3R,4S)-4-hydroxy-3-(hydroxymethyl)-2-methylenecyclopentyl)-1,9-dihydro-6H-purin-6-one hydrate 2-amino-9-((1R,3R,4S)-4-hydroxy-3-(hydroxymethyl)-2-methylenecyclopentyl)-1,9-dihydro-6H-purin-6-one hydrate 1367369-78-5 C12H15N5O3
Moxifloxacin Impurity 6 Moxifloxacin Impurity 6 849770-49-6 C25H29BFN3O8
beta-D-Hydroxymethoxymethylanthraquinonyl glucopyranoside beta-D-Hydroxymethoxymethylanthraquinonyl glucopyranoside 29013-18-1 C22H22O10
Sitafloxacin Impurity A Sitafloxacin Impurity A 500295-83-0 C16H16FN3O3
1-benzylpiperidin-4-yl [1,1'-biphenyl]-2-ylcarbamate 1-benzylpiperidin-4-yl [1,1'-biphenyl]-2-ylcarbamate 171723-80-1 C25H26N2O2
Merariveron impurity C Merariveron impurity C
4H-Inden-4-one, 1-[1,5-dimethyl-5-[(triethylsilyl)oxy]hexyl]octahydro-7a-methyl-, [1R-[1α(R*),3aβ,7aα]]- 4H-Inden-4-one, 1-[1,5-dimethyl-5-[(triethylsilyl)oxy]hexyl]octahydro-7a-methyl-, [1R-[1α(R*),3aβ,7aα]]- 144848-24-8 C24H46O2Si
Cefazoline Impurity A Cefazoline Impurity A
Rifaximin impurity A Rifaximin impurity A
methyl (Z)-3-(((4-(2-(4-(2-((4-((((Z)-6-(methoxymethyl)-2-oxoindolin-3-ylidene)(phenyl)methyl)amino)phenyl)(methyl)amino)-2-oxoethyl)piperazin-1-yl)-N-methylacetamido)phenyl)amino)(phenyl)methylene)-2-oxoindoline-6-carboxylate methyl (Z)-3-(((4-(2-(4-(2-((4-((((Z)-6-(methoxymethyl)-2-oxoindolin-3-ylidene)(phenyl)methyl)amino)phenyl)(methyl)amino)-2-oxoethyl)piperazin-1-yl)-N-methylacetamido)phenyl)amino)(phenyl)methylene)-2-oxoindoline-6-carboxylate 2097505-01-4 C56H54N8O7
Roxithromycin EP Impurity J Roxithromycin EP Impurity J 134834-12-1 C40H73ClN2O14
Venlafaxine D6 Venlafaxine D6 940297-06-3 C17H27NO2
Levosimendan Impurity 3 Levosimendan Impurity 3
Sofosbuvir Impurity29 Sofosbuvir Impurity29
Acarbose Impurity A Acarbose Impurity A
Levosimendan Impurity 1 Levosimendan Impurity 1 1643439-59-1 C22H23N7O2
Atomoxetine EP Impurity F Atomoxetine EP Impurity F
Quetiapine EP Impurity Q Quetiapine EP Impurity Q
Cefoxitin Impurity Cefoxitin Impurity
Acemetacin Impurity Acemetacin Impurity
ethyl (S)-6,7,8-trifluoro-1-(1-hydroxypropan-2-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylate ethyl (S)-6,7,8-trifluoro-1-(1-hydroxypropan-2-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylate 110548-03-3 C15H14F3NO4
ethyl (E)-2-(2-(4-methoxyphenyl)hydrazono)propanoate ethyl (E)-2-(2-(4-methoxyphenyl)hydrazono)propanoate 4792-57-8 C12H16N2O3
ACT 373898 ACT 373898 1433875-14-9 C15H17BrN4O5S
Reaxys ID: 3623864 Reaxys ID: 3623864 129385-61-1 C17H10ClNO3
Olaparib Impurity D Olaparib Impurity D
1-methylpyrrolidin-3-yl 2-(cyclopentyloxy)-2-phenylacetate 1-methylpyrrolidin-3-yl 2-(cyclopentyloxy)-2-phenylacetate C18H25NO3
Cabozantinib impurity A Cabozantinib impurity A
Loratadine Impurity 2 Loratadine Impurity 2 C22H23ClN2O2
Metoprolol Impurity 1 Metoprolol Impurity 1 C15H25NO3
Pitavastatin Impurity 1 Pitavastatin Impurity 1
Trifluridine Impurity 3 Trifluridine Impurity 3
Vonoprazan Impurity 1 Vonoprazan Impurity 1
Vonoprazan Impurity 4 Vonoprazan Impurity 4
Cinacalcet impurity K Cinacalcet impurity K
Valsartan Impurity 13 Valsartan Impurity 13 C24H29N5O3
Valsartan Impurity 27 Valsartan Impurity 27 C24H29N5O3
Etoricoxib Impurity 6 Etoricoxib Impurity 6
Ezetimibe impurity 3 Ezetimibe impurity 3
Apremilast Impurity C Apremilast Impurity C
bis(1-benzylpiperidin-3-yl) 2,6-dimethyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate bis(1-benzylpiperidin-3-yl) 2,6-dimethyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate 166808-97-5 C16H16N2O6
Ticagrelor Imp.J Ticagrelor Imp.J 2248538-63-6 C14H20ClN5O4S
Vortioxetine Imp.E Vortioxetine Imp.E 508233-77-0 C18H22N2S
(3R,4S)-1-(4-fluorophenyl)-3-((S)-3-hydroxy-3-(p-tolyl)propyl)-4-(4-hydroxyphenyl)azetidin-2-one (3R,4S)-1-(4-fluorophenyl)-3-((S)-3-hydroxy-3-(p-tolyl)propyl)-4-(4-hydroxyphenyl)azetidin-2-one 1700622-07-6 C25H24FNO3
Topiroxostat Impurity 1 Topiroxostat Impurity 1
Oxcarbazepine Impurity3 Oxcarbazepine Impurity3
Mosapride  IMP Mosapride IMP
4-Ethoxy Paroxetine 4-Ethoxy Paroxetine 606968-04-1 C21H25NO4
Methylene-Bis Paroxetine Methylene-Bis Paroxetine 1292846-10-6 C39H40F2N2O6
Trelagliptin Impurity X Trelagliptin Impurity X 1938080-44-4 C18H22FN5O3
Afatinib Impurity 19 Afatinib Impurity 19 1245555-42-3 C21H21ClFN5O2
Cefoxitin Sodium impurity Cefoxitin Sodium impurity
(1S,2S,3S)-Aprepitant (1S,2S,3S)-Aprepitant 1242175-40-1 C23H21F7N4O3
2-{[(methoxycarbonylmethyl)(toluene-4-sulfonyl)amino]methyl}-4-phenoxybenzoic acid methyl ester 2-{[(methoxycarbonylmethyl)(toluene-4-sulfonyl)amino]methyl}-4-phenoxybenzoic acid methyl ester 1455091-06-1 C25H25NO7S
Benzene, 1-chloro-2-(dimethoxymethyl)- Benzene, 1-chloro-2-(dimethoxymethyl)- 70380-66-4 C9H11ClO2
(R)-1-chloro-3-{[(4-chlorophenyl)methylene]amino}propan-2-ol (R)-1-chloro-3-{[(4-chlorophenyl)methylene]amino}propan-2-ol 1450915-93-1 C10H11Cl2NO
3-(trifluoromethyl)-6,7-dihydro-[1,2,4]triazolo[4,3-a]pyrazin-8(5H)-one 3-(trifluoromethyl)-6,7-dihydro-[1,2,4]triazolo[4,3-a]pyrazin-8(5H)-one 877402-45-4 C6H5F3N4O
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