| Identification | More | [Name]
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride | [CAS]
1185-53-1 | [Synonyms]
2-AMINO-2-(HYDROXYMETHYL)-1,3-PROPANEDIOL, HYDROCHLORIDE
MOLE-READY-TRIS
SPECTRIS(R) HYDROCHLORIDE
SPECTRIS(TM) HYDROCHLORIDE
TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE
TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE
TRIS-HCL
TRIS-HCL BUFFER
TRIS HYDROCHLORIDE
TRIS(HYDROXYMETHYL)AMINOMETHANE, HCL
TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE
TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE BUFFER SOLUTION
TRIS(HYDROXYMETHYL)METHYLAMINE HYDROCHLORIDE
TRIS(HYDROXYMETHYL)METHYLAMMONIUM CHLORIDE
TRISMAT(R) HYDROCHLORIDE
TRISMAT(TM) HYDROCHLORIDE
TRIZMA HYDROCHLORIDE
TRIZMA(R) HYDROCHLORIDE
TROMETAMOL HYDROCHLORIDE
TROMETHAMINE HYDROCHLORIDE | [EINECS(EC#)]
214-684-5 | [Molecular Formula]
C4H12ClNO3 | [MDL Number]
MFCD00012590 | [Molecular Weight]
157.6 | [MOL File]
1185-53-1.mol |
| Chemical Properties | Back Directory | [Appearance]
White crystalline powder | [Melting point ]
150-152 °C
| [Boiling point ]
225℃[at 101 325 Pa] | [density ]
1.05 g/mL at 20 °C
| [vapor pressure ]
0Pa at 20℃ | [storage temp. ]
Store at RT. | [solubility ]
H2O: 4 M at 20 °C, clear, colorless
| [form ]
crystalline
| [color ]
clear colorless (40 % (w/w) in H2O) solution
| [PH]
2.5-4.0 (25℃, 4M in H2O) | [PH Range]
7.0 - 9.0 | [pka]
8.1(at 25℃) | [Stability:]
Stable. Incompatible with strong oxidizing agents, bases, moisture. | [Water Solubility ]
SOLUBLE | [Sensitive ]
Hygroscopic | [λmax]
λ: 260 nm Amax: 0.12
λ: 280 nm Amax: 0.08 | [Detection Methods]
T,NMR | [BRN ]
3675235 | [InChIKey]
QKNYBSVHEMOAJP-UHFFFAOYSA-N | [LogP]
-3.6 at 20℃ | [CAS DataBase Reference]
1185-53-1(CAS DataBase Reference) | [EPA Substance Registry System]
1185-53-1(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing . | [WGK Germany ]
3
| [RTECS ]
TY2900000 | [F ]
3 | [TSCA ]
Yes | [HS Code ]
29221980 |
| Hazard Information | Back Directory | [Description]
Abbreviated as Tris HCl (1185-53-1), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0. The compound is instrumental in preparing Laemmli buffer, one of the most widely used SDS-PAGE buffers, and also finds application in various custom running and loading buffers, frequently in combination with glycine and SDS. Lysis, the initial step of DNA extraction, involves a buffer containing Tris and EDTA (ethylenediaminetetraacetic acid). In this process, EDTA binds to divalent cations such as calcium and magnesium. Due to their role in maintaining cell membrane integrity, their removal by EDTA leads to membrane destabilization. Tris plays a key role as the primary buffering component, maintaining the pH of the buffer at a stable point, typically around 8.0. Additionally, Tris may interact with the LPS (lipopolysaccharide) in the membrane, contributing to further membrane destabilization. This underscores Tris HCl's significance in various laboratory procedures.
| [Chemical Properties]
White crystalline powder | [Uses]
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode;For Tris buffers, pH increases about 0.03 unit/°C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. | [Application]
TRIS hydrochloride is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer. | [Preparation]
The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid. | [General Description]
Trizma? hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution. | [Biological Activity]
Commonly used laboratory reagent | [Purification Methods]
Crystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.] |
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