| Identification | More | [Name]
Tricine | [CAS]
5704-04-1 | [Synonyms]
2-AMINO-2-(HYDROXYMETHYL)-1,3-PROPANEDIOL, HYDROCHLORIDE
LABOTEST-BB LT00454966
MOLE-READY-TRIS
N-[2-HYDROXY-1,1-BIS(HYDROXYMETHYL)ETHYL]GLYCINE
N-[TRIS(HYDROXYMETHYL)METHYL]GLYCINE
SPECTRIS(R) HYDROCHLORIDE
SPECTRIS(TM) HYDROCHLORIDE
TRICINE
TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE
TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE
TRIS-HCL
TRIS-HCL BUFFER
TRIS HYDROCHLORIDE
TRIS(HYDROXYMETHYL)AMINOMETHANE, HCL
TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE
TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE BUFFER SOLUTION
TRIS(HYDROXYMETHYL)METHYLAMINE HYDROCHLORIDE
TRIS(HYDROXYMETHYL)METHYLAMMONIUM CHLORIDE
TRIS(HYDROXYMETHYL) METHYLGLYCINE
TRISMAT(R) HYDROCHLORIDE | [EINECS(EC#)]
214-684-5 | [Molecular Formula]
C6H13NO5 | [MDL Number]
MFCD00012590 | [Molecular Weight]
179.17 | [MOL File]
5704-04-1.mol |
| Chemical Properties | Back Directory | [Appearance]
White/clear crystalline powder | [Melting point ]
186-188 °C (dec.)
| [Boiling point ]
311.69°C (rough estimate) | [density ]
1.05 g/mL at 20 °C
| [vapor pressure ]
0.002-0.004Pa at 20-25℃ | [refractive index ]
1.4240 (estimate) | [storage temp. ]
Store at RT. | [solubility ]
H2O: 4 M at 20 °C, clear, colorless
| [form ]
crystalline
| [color ]
clear colorless (40 % (w/w) in H2O) solution
| [Odor]
Odorless | [PH]
4.0-6.0 (1M in H2O) | [PH Range]
7.4 - 8.8 | [pka]
8.1(at 25℃) | [Stability:]
Stable. | [Water Solubility ]
soluble | [Merck ]
14,9651 | [BRN ]
1937804 | [LogP]
-2.87 at 25℃ | [CAS DataBase Reference]
5704-04-1(CAS DataBase Reference) | [EPA Substance Registry System]
5704-04-1(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing .
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection . | [WGK Germany ]
3
| [F ]
3 | [TSCA ]
Yes | [HS Code ]
29052900 |
| Questions And Answer | Back Directory | [Descriptioin]
Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.3
| [Reference]
- S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
- H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379
|
| Hazard Information | Back Directory | [Chemical Properties]
White/clear crystalline powder | [Uses]
Biological buffer. | [Definition]
ChEBI: N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 ℃. It is functionally related to a member of tris and a glycine. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate. | [General Description]
Trizma? and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood. | [Biological Activity]
Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy. | [Purification Methods]
Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.] |
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