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Bioresource Technology

Bioresource Technology

IF: 9.69
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Production of capsaicinoid nonivamide from plant oil and vanillylamine via whole-cell biotransformation

Published:21 October 2023 DOI: 10.1016/j.biortech.2023.129883 PMID: 37871741
Jianzhong Ge , Jie Zhang , Xiaolu Wang , Yingguo Bai , Yaru Wang , Yuan Wang , Tao Tu , Xing Qin , Xiaoyun Su , Huiying Luo , Bin Yao , Huoqing Huang

Abstract

Capsaicinoids are mostly derived from chili peppers and have widespread applications in food, feed, and pharmacology. Compared with plant extraction, the use of microbial cell factories for capsaicinoids production is considered as a more efficient approach. Here, the biotransformation of renewable plant oil and vanillylamine into capsaicinoid nonivamide was investigated. Nonivamide biosynthesis using nonanoic acid and vanillylamine as substrates was achieved in Escherichia coli by heterologous expression of genes encoding amide-forming N-acyltransferase and CoA-ligase. Through increasing nonanoic acid tolerance of chassis cell, screening key enzymes involved in nonivamide biosynthesis and optimizing biotransformation conditions, the nonivamide titer reached 0.5?g/L. By further integrating a route for conversion of oleic acid to nonanoic acid, nonivamide biosynthesis was finally achieved using olive oil and vanillylamine as substrates, yielding a titer of approximately 10.7?mg/L. Results from this study provide valuable information for constructing highly efficient cell factories for the production of capsaicinoid compounds.

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