天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

ChemicalBook >> journal list >> Bioresource Technology >>article
Bioresource Technology

Bioresource Technology

IF: 9.69
Download PDF

Investigating enhancement of protease and lysozyme combination pretreatment on hydrolysis of sludge organics under humic acid inhibition

Published:4 December 2024 DOI: 10.1016/j.biortech.2024.131928 PMID: 39643052
Xiang Zou,?Junguo He,?Xinlei Pan,?Qiupeng Cai,?Shengye Duan,?Yijie Zhong,?Xinxin Cui,?Jie Zhang

Abstract

This study investigated the impact of humic acid (HA) on enzymatic pretreatment efficiency, focusing on sludge properties and HA molecular structure. The results showed that enzymatic pretreatment alleviates HA inhibition, improving hydrolysis efficiency. In the presence of HA, soluble proteins and polysaccharides in the enzyme-cocktail group reached 27.7?mg/L and 23.9?mg/L, 1.4 and 1.3 times higher than the blank group, respectively. The enzyme-cocktail group also had the highest soluble DNA concentration (19.4?mg/L) and the lowest viable cell proportion (69.3?%), indicating effective cell lysis. Enzyme-cocktail pretreatment reduced electrostatic repulsion, enhancing the mobility of extracellular organics. Enzyme interactions with HA released internal hydrolases and decreased amide groups on the HA surface, increasing the availability of biodegradable substrates. Overall, enzymatic pretreatment proves effective in mitigating HA-induced inhibition, thereby improving sludge biodegradation and enhancing carbon recovery in anaerobic fermentation.

Substances (2)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Lysozyme 12650-88-3 C125H196N40O36S2 429 suppliers $29.40-$2580.00
DISPASE 78990-62-2 58 suppliers Inquiry