天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

ChemicalBook >> journal list >> Journal of microbiology and biotechnology >>article
Journal of microbiology and biotechnology

Journal of microbiology and biotechnology

IF: 2.5
Download PDF

Production of chlorphenesin galactoside by whole cells of β-galactosidase-containing Escherichia coli.

Published:28 June 2013 DOI: 10.4014/jmb.1211.11009 PMID: 23676917
Sang-Eun Lee, Hyang-Yeol Lee, Kyung-Hwan Jung

Abstract

We investigated the transgalactosylation reaction of chlorphenesin (CPN) using β-galactosidase (β-gal)-containing Escherichia coli (E. coli) cells, in which galactose from lactose was transferred to CPN. The optimal CPN concentration for CPN galactoside (CPN-G) synthesis was observed at 40 mM under the conditions that lactose and β-gal (as E. coli cells) were 400 g/l and 4.8 U/ml, respectively, and the pH and temperature were 7.0 and 40oC, respectively. The time-course profile of CPN-G synthesis under these optimal conditions showed that CPN-G synthesis from 40 mM CPN reached a maximum of about 27 mM at 12 h. This value corresponded to an about 67% conversion of CPN to CPN-G, which was 4.47-5.36-fold higher than values in previous reports. In addition, we demonstrated by thin-layer chromatography to detect the sugar moiety that galactose was mainly transferred from lactose to CPN. Liquid chromatography-mass spectrometry revealed that CPN-G and CPN-GG (CPN galactoside, which accepted two galactose molecules) were definitively identified as the synthesized products using β-gal-containing E. coli cells. In particular, because we did not use purified β-gal, our β-gal-containing E. coli cells might be practical and cost-effective for enzymatically synthesizing CPN-G. It is expected that the use of β-gal-containing E. coli will be extended to galactose derivatization of other drugs to improve their functionality.

Substances (6)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Chlorphenesin 104-29-0 C9H11ClO3 520 suppliers $8.00-$847.00
β-Galactosidase 9031-11-2 NULL 261 suppliers $45.00-$3130.00
D-Lactose monohy 14641-93-1 C12H22O11 173 suppliers Inquiry
D-(+)-GALACTOSE 10257-28-0 C6H12O6 81 suppliers $138.00-$490.00
ESCHERICHIA COLI 24 suppliers $257.00-$3911.12
4-(P-CHLORO-1-CYANOBENZYL)-(2H)-PHTHALAZINONE 160748-31-2 C16H10ClN3O 7 suppliers Inquiry

Similar articles

IF:11.6

The discovery of β-galactosidase

Trends in Biochemical Sciences R.J. Rouwenhorst, J.T. Pronk,etc Published: 1 October 1989
IF:4.3

Metabolic engineering of Escherichia coli for high-level production of benzyl acetate from glucose.

Microbial Cell Factories Qin Ke,?Chang Liu,etc Published: 3 September 2024
IF:0

1,2-Octanediol deracemization by stereoinversion using whole cells

Journal of Molecular Catalysis B-enzymatic Lu S. Chen , Simone M. Mantovani ,etc Published: 1 July 2008