天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

ChemicalBook >> journal list >> Cell reports >>article
Cell reports

Cell reports

IF: 7.5
Download PDF

DCP1A, a MEK substrate, regulates the self-renewal and differentiation of mouse embryonic stem cells

Published:12 December 2024 DOI: 10.1016/j.celrep.2024.115058 PMID: 39671288
Jiayu Yu,?Nannan Zhao,?Yuying Wang,?Nan Ding,?Zhenchang Guo,?Zichan He,?Qingye Zhang,?Jingai Zhang,?Xiaoqiong Yang,?Ming Zhang,?Xiaoling Du,?Kai Zhang,?Lingyi Chen

Abstract

Mitogen-activated extracellular signal-regulated kinase (MEK) inhibitors are widely applied to maintain pluripotency, while prolonged MEK inhibition compromises the developmental potential of mouse embryonic stem cells (ESCs). To understand the mechanism of MEK in pluripotency maintenance, we first demonstrated that MEK regulates gene expression at post-transcriptional steps. Consistently, many of the 66 MEK substrates identified by quantitative phosphoproteomics analysis are involved in RNA processing. We further confirmed that MEK1 phosphorylates S563 of DCP1A, an mRNA decapping cofactor and processing body (P body) component. DCP1A, as well as two other P body components, EDC4 and DCP2, are required for the self-renewal and differentiation of ESCs, indicating the role of P bodies in ESCs. Dephosphorylation of DCP1A S563 facilitates both self-renewal and differentiation of ESCs through promoting P body formation and RNA storage. In summary, our study identified 66 MEK substrates supporting the extracellular signal-regulated kinase (ERK)-independent function of MEK and revealed that DCP1A, phosphorylated by MEK, regulates ESC self-renewal and differentiation through modulating P body formation.

Substances (9)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Dimethyl sulfoxide 67-68-5 C2H6OS 1348 suppliers $15.00-$120900.00
L-Glutamine 56-85-9 C5H10N2O3 1143 suppliers $5.00-$10696.00
Formaldehyde 50-00-0 CH2O 855 suppliers $10.00-$1640.00
Paraformaldehyde 30525-89-4 (CH2O)x 736 suppliers $18.00-$1190.00
Phenylmethylsulfonyl fluoride 329-98-6 C7H7FO2S 413 suppliers $5.00-$2530.00
Proteinase K 39450-01-6 C29H27N2O12P 412 suppliers $45.48-$9570.00
Doxycycline 564-25-0 C22H24N2O8 339 suppliers Inquiry
PD 0325901 391210-10-9 C16H14F3IN2O4 208 suppliers $8.00-$1680.00
PUROMYCIN 53-79-2 C22H29N7O5 91 suppliers $73.60-$1200.00

Similar articles

IF:10

Localized Hydrogel Microspheres for Osteoarthritis Treatment: Recruitment and Differentiation of Stem Cells

Advanced Healthcare Materials Qiming Pang, Zhuolin Chen,etc Published: 28 November 2024
IF:0.8

Establishment of a CPAMD8-GFP reporter human embryonic stem cell line, IBBDe001-B, using CRISPR/Cas9 editing

Stem cell research Shun Zhang, Jiahang Li,etc Published: 22 November 2024