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Stem cell research

Stem cell research

IF: 0.8
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Establishment of a CPAMD8-GFP reporter human embryonic stem cell line, IBBDe001-B, using CRISPR/Cas9 editing

Published:22 November 2024 DOI: 10.1016/j.scr.2024.103615
Shun Zhang, Jiahang Li, Shasha Li

Abstract

The human CPAMD8 gene encodes proteins in the A2M/C3 (alpha-2-macroglobulin/complement 3) family, predominantly expressed in the distal tips of the retinal neuroepithelium that form the iris and ciliary body. Mutations in CPAMD8 have been linked to anterior segment dysplasia and congenital glaucoma. Using CRISPR/Cas9 editing, we inserted a 3*EAAAK-EGFP fluorescent tag into the CPAMD8 gene, enabling real-time observation of its expression and providing insights into its biological functions. The resulting gene-edited cell line retained normal stem cell morphology and karyotype, expressed essential pluripotency markers, and exhibited differentiation potential.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Ethylenediaminetetraacetic acid 60-00-4 C10H16N2O8 1172 suppliers $10.00-$6397.62
Triton X-100 9002-93-1 (C2H4O)nC14H22O 535 suppliers $18.00-$610.00
4',6-Diamidino-2-phenylindole dihydrochloride 28718-90-3 C16H16ClN5 302 suppliers $14.10-$4800.00
Y-27632 DIHYDROCHLORIDE 146986-50-7 C14H21N3O 172 suppliers $43.00-$2691.10

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