天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

ChemicalBook >> journal list >> Biosensors >>article

Biosensors

Download PDF

D-Glucose-Mediated Gold Nanoparticle Fabrication for Colorimetric Detection of Foodborne Pathogens

Published:1 June 2024 DOI: 10.3390/bios14060284
Seo Yeon Park, Rajamanickam Sivakumar, Nae Yoon Lee

Abstract

Gold nanoparticle (AuNP) fabrication via the oxidation of D-glucose is applied for detecting two foodborne pathogens, Enterococcus faecium (E. faecium) and Staphylococcus aureus (S. aureus). D-glucose is used as a reducing agent due to its oxidation to gluconic acid by sodium hydroxide (NaOH), resulting in the formation of AuNPs. Based on this mechanism, we develop AuNP-based colorimetric detection in conjunction with loop-mediated isothermal amplification (LAMP) for accurately identifying the infectious bacteria. Here, Au+ ions bind to the base of double-stranded DNA. In the presence of D-glucose and NaOH, the LAMP amplicon-Au+ complex maintains its bound state at 65 °C for 10 min while it is reduced to AuNPs in a dispersed form, exhibiting a red color. We aimed to pre-mix D-glucose with LAMP reagents before amplification and induce successful colorimetry without inhibiting amplification to simplify the experimental process and decrease the reaction time. Therefore, the entire process, including LAMP and colorimetric detection, is accomplished in approximately 1 h. The limit of detection of E. faecium and S. aureus is confirmed using the introduced method as 101 CFU/mL and 100 fg/μL, respectively. We expect that colorimetric detection using D-glucose-mediated AuNP synthesis offers an application for simple and immediate molecular diagnosis.

Substances (4)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Sodium hydroxide 1310-73-2 NaOH 1210 suppliers $14.00-$16472.00
D(+)-Glucose 50-99-7 C6H12O6 1113 suppliers $6.00-$26950.00
GOLD 7440-57-5 Au 288 suppliers $43.30-$9840.00
AUNP12 acetate,AUNP 12 acetate 1 suppliers Inquiry

Similar articles

IF:3.9

Detection of β-lactamase-mediated resistance

Journal of Antimicrobial Chemotherapy D. Livermore,?Derek F. J. Brown,etc Published: 1 July 2001