喜樹(shù)堿|T1123

價(jià)格	￥140	￥220
包裝	20mg	50mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-02

QQ交談微信洽談

產(chǎn)品詳情

中文名稱:喜樹(shù)堿	英文名稱:Camptothecin
CAS:7689-03-4	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.52%	產(chǎn)品類別: 抑制劑
貨號(hào): T1123

Product Introduction

Bioactivity

名稱	Camptothecin
描述	Camptothecin (CPT) belongs to the alkaloid group of natural products and is a specific DNA topoisomerase I (Topo I) inhibitor (IC50=679 nM) with specificity. Camptothecin has antitumor activity and induces apoptosis.
細(xì)胞實(shí)驗(yàn)	Tumor cells are plated in 100 μL of medium in 96-well microtiter plates at a density of 1500 to 4000 cells per well and allowed to adhere overnight. Cells are incubated with Camptothecin for 48 hours and then with fresh medium for 48 hours. Camptothecin at each concentration is added in quadruplicate. Following a 4-hour incubation of treated cells with MTT, the reduced dye product is extracted from the cells with 0.2 mL of DMSO followed by 50 μL of Sorensen's buffer. The plates are shaken briefly, and the absorbance at 570 nm is read and quantitated. Curves are fitted to the MTT assay data using a four-parameter logistic equation.(Only for Reference)
激酶實(shí)驗(yàn)	Topoisomerase I Cleavable Complex Assay: Topoisomerase I is isolated from calf thymus and is devoid of topoisomerase II. All reactions are carried out in 10 mL volumes of reaction buffer (50 mM Tris-HCl, pH 7.5, 100 mM KCl, 0.5 mM EDTA, and 30 pg/mL BSA) in microtiter plates. Camptothecin is dissolved in DMSO at 10 mg/mL and serially diluted in 96-well microtiter plates to which the 32P end-labeled pBR322 DNA and topoisomerase enzyme are added. The reaction mixture is incubated at room temperature for 30 min and then the reaction stopped by adding 2 mL of a mixture of sodium dodecyl sulfate and proteinase K (1.6% and 0.14 mg/mL final concentrations, respectively). The plates are heated at 50 °C for 30 min, 10 mL of standard stop mixture containing 0.45 N NaOH is added in order to generate single-stranded DNA, and the samples are electrophoresed in 1.5% agarose gels in TBE buffer. Gels are blotted on nitrocellulose paper, dried, and exposed to X-ray film. The units of cleavage are calculated from the autoradiographs and plotted against the log drug concentration. The IC50 values are then obtaine
體外活性	方法：八株 TNBC 細(xì)胞系用 Camptothecin (0.1-5 μM) 處理 72 h，使用 PrestoBlue 檢測(cè)細(xì)胞活力。結(jié)果：Camptothecin 抑制 MCF7、HCC1428、HCC1419、HCC202、MDA453、MDA231、Sum149、BT549 細(xì)胞活力，IC50 值分別為 0.089/0.448/0.067/0.481/0.058/0.040/0.065/0.056 μM。[1] 方法：肺癌細(xì)胞 H1299 和 H460 用 Camptothecin (0.5-5 μM) 處理 16 h，使用 wound-healing assay 檢測(cè)細(xì)胞遷移情況。結(jié)果：Camptothecin 抑制 H1299 和 H460 細(xì)胞遷移，但沒(méi)有劑量依賴性作用。[2]
體內(nèi)活性	方法：為檢測(cè)體內(nèi)抗腫瘤活性，將 Camptothecin (0.15-1.2 mg/kg) 和 doxorubicin (0.25-2 mg/kg) 靜脈注射給攜帶三陰性乳腺癌腫瘤 MDA-MB-231 的 athymic nu/n 小鼠，每?jī)商煲淮?，給藥四次。結(jié)果：觀察到腫瘤生長(zhǎng)的劑量依賴性減少，在 0.5 mg/kg DOX + 0.3 mg/kg CPT 下減少 40.8% ，在 1.5 mg/kg DOX + 0.9 mg/kg CPT 下減少 93%。最高的測(cè)試劑量 (2 mg/kg DOX + 1.2 mg/kg CPT) 在第 44 天完全停止了腫瘤的生長(zhǎng)。[3] 方法：為研究對(duì)肥胖的作用，將 Camptothecin (1 mg/kg，0.1% Tween 80) 口服給藥給肥胖小鼠，每天一次，持續(xù)三天。結(jié)果：口服 Camptothecin 可增加飲食誘導(dǎo)肥胖 (DIO) 小鼠和遺傳 ob/ob 小鼠的循環(huán) GDF15 水平。與 GDF15 的厭食作用一致，Camptothecin 抑制食物攝入，從而降低肥胖小鼠的體重、血糖和肝臟脂肪含量。[4]
存儲(chǔ)條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	10% DMSO+40% PEG300+5% Tween 80+45% Saline : 0.5 mg/mL (1.44 mM), Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO : 3.48 mg/mL (10 mM)
關(guān)鍵字	Apoptosis \| NSC100880 \| HIF-1α \| Inhibitor \| Antibiotic \| ADC Payload \| DNA topoisomerase I \| microRNAs \| NSC 100880 \| Top1 \| miRNA \| inhibit \| Camptothecin \| Topoisomerase \| alkaloid \| miRN \| Influenza Virus \| MicroRNA \| Fungal \| ADC Cytotoxin
相關(guān)產(chǎn)品	L-Glutamic acid \| Dehydroacetic acid sodium \| Doxycycline \| Neomycin sulfate \| Geraniol \| Dextran sulfate sodium salt (MW 4500-5500) \| Stavudine \| Ampicillin sodium \| L-Ascorbic acid \| Sulfamethoxazole sodium \| Kanamycin sulfate \| Sodium 4-phenylbutyrate
相關(guān)庫(kù)	經(jīng)典已知活性庫(kù) \| 抗癌活性化合物庫(kù) \| 生物堿類天然產(chǎn)物庫(kù) \| 天然產(chǎn)物庫(kù) \| 藥物功能重定位化合物庫(kù) \| 抗癌臨床化合物庫(kù)

關(guān)鍵字：喜樹(shù)堿|||NSC-100880|||CPT|||Campathecin|||(S)-(+)-Camptothecin|TargetMol

公司簡(jiǎn)介

上海陶術(shù)生物科技有限公司為美國(guó)Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國(guó)波士頓、德國(guó)慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實(shí)體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個(gè)性化定制合成；從對(duì)明確靶點(diǎn)的分子篩選到對(duì)明確分子的多靶點(diǎn)篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過(guò)在中國(guó)市場(chǎng)五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過(guò)五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊(cè)資本	566.2651萬(wàn)人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營(yíng)行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營(yíng)模式	貿(mào)易,試劑,定制,服務(wù)

TargetMol中國(guó)（陶術(shù)生物）

VIP 12年

公司成立：12年
注冊(cè)資本：566.2651萬(wàn)人民幣
企業(yè)類型：有限責(zé)任公司(自然人投資或控股)
主營(yíng)產(chǎn)品：小分子抑制劑，藥物篩選化合物庫(kù)，天然產(chǎn)物，活性分子化合物等
公司地址：上海市閘北區(qū)江場(chǎng)三路28號(hào)4樓

進(jìn)入店鋪

詢盤

店內(nèi)推薦

2'-脫氧胸苷5'-三磷酸|T1663

￥296

咪唑斯汀|T0118

￥243

齊墩果烷三萜化合物|T2915

￥667