銀杏素
| 中文名稱 | 銀杏素 |
|---|---|
| 中文同義詞 | GINKGETIN 銀杏雙黃酮;白果雙黃酮(銀杏雙黃酮,銀杏黃素,銀杏素);白果雙黃酮,銀杏雙黃酮,銀杏黃素;銀杏黃酮甙;銀杏雙黃酮,銀杏黃素,銀杏素;銀杏素;銀杏雙黃酮/白果雙黃酮;銀杏雙黃酮, 來(lái)源于銀杏葉 |
| 英文名稱 | GINKGETIN |
| 英文同義詞 | 4',5,5'',7-Tetrahydroxy-4''',7''-dimethoxy-8,3'''-biflavone;5,7-Dihydroxy-8-[2-methoxy-5-(5-hydroxy-7-methoxy-4-oxo-4H-1-benzopyran-2-yl)phenyl]-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one;5,7-Dihydroxy-8-[5-(5-hydroxy-7-methoxy-4-oxo-4H-1-benzopyran-2-yl)-2-methoxyphenyl]-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one;GINKGETIN(RG)(PLEASE CALL);GINKGETIN;Ginkgetin, >98%;4H-1-Benzopyran-4-one,5,7-dihydroxy-8-[5-(5-hydroxy-7-methoxy-4-oxo-4H-1-benzopyran-2-yl)-2-methoxyphenyl]-2-(4-hydroxyphenyl)-;Ginkgetin, 98%, from Ginkgo biloba L. |
| CAS號(hào) | 481-46-9 |
| 分子式 | C32H22O10 |
| 分子量 | 566.51 |
| EINECS號(hào) | |
| 相關(guān)類別 | 中草藥成分;中藥對(duì)照品;標(biāo)準(zhǔn)品;對(duì)照品;植物提取物;分析試劑標(biāo)準(zhǔn)品;對(duì)照品-中藥對(duì)照品;黃酮;標(biāo)準(zhǔn)品 -中藥標(biāo)準(zhǔn)品;標(biāo)準(zhǔn)品，對(duì)照品;通用生化試劑-天然產(chǎn)物 |
| Mol文件 | 481-46-9.mol |
| 結(jié)構(gòu)式 |  |
銀杏素 性質(zhì)
| 熔點(diǎn) | 297 °C(Solv: acetone (67-64-1)) |
|---|---|
| 沸點(diǎn) | 863.7±65.0 °C(Predicted) |
| 密度 | 1.506±0.06 g/cm3(Predicted) |
| 儲(chǔ)存條件 | Store at -20°C |
| 溶解度 | DMSO:64.0(最大濃度 mg/mL);112.97(最大濃度 mM) DMSO:PBS (pH 7.2) (1:7):0.12(最大濃度 mg/mL);0.21(最大濃度 mM） |
| 酸度系數(shù)(pKa) | 6.18±0.40(Predicted) |
| 形態(tài) | 粉末 |
| 顏色 | 淺黃色 |
| LogP | 4.450 (est) |
|
Wnt 5.92 μM (IC 50 ) |
Ginkgetin (2.5-20 μM; 48 h) inhibits the growth of Daoy and D283 cell lines, and induces G
2
/M cell cycle arrest in Daoy cells.
Ginkgetin (20-40 μM; 24 h) significantly activates the apoptosis of osteosarcoma cells in a concentration-dependent manner.
Ginkgetin (10-20 μM; 3-24 h) down-regulated the expression of Wnt target genes without affecting the expression of β-catenin in medulloblastoma cells.
Ginkgetin (1-10 μM; 24 or 48 h) significantly inhibits the VEGF-induced endothelial cell proliferation, migration, and wound recovery in a concentration-dependent manner.
Ginkgetin (5-10 μM; 48 h) induces autophagy responsible for cell death in A549.
Cell Viability Assay
| Cell Line: | Daoy and D283 cell lines |
| Concentration: | 2.5, 5, 10, 20 μM |
| Incubation Time: | 48 hours |
| Result: | Inhibited the cell growth, with IC 50 s of 14.65 and 15.81 μM for Daoy and D283 cells, respectively. |
Apoptosis Analysis
| Cell Line: | Osteosarcoma cells |
| Concentration: | 20, 30, 40 μM |
| Incubation Time: | 24 hours |
| Result: | Markedly induced the apoptosis of osteosarcoma cells in a concentration-dependent manner. |
Cell Cycle Analysis
| Cell Line: | Daoy cells |
| Concentration: | 2.5, 5, 10, 20 μM |
| Incubation Time: | 24 hours |
| Result: | Increased G 2 /M phase, compared with that of control, indicating a G 2 /M cell phase arrest. |
Cell Cycle Analysis
| Cell Line: | Daoy and D283 cell lines |
| Concentration: | 10, 20 μM |
| Incubation Time: | 3, 6, 12, 24 hours |
| Result: |
Attenuated the expression of Wnt target genes, Axin2, cyclin D1 and survivin at 20 μM for 24 h in Daoy cells.
Unaffected the level of total β-catenin and diminished the level of β-catenin phosphorylation in a time- and concentration-dependent manner. |
Ginkgetin (25-100 mg/kg; i.p. 2 hours after the onset of ischemia) exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway.
Ginkgetin (30 mg/kg; intragastrically once per day for 42 d) suppresses tumor growth in A549 cells bearing nude mice.
| Animal Model: | Male Sprague-Dawley rats (200-220 g) |
| Dosage: | 25, 50, 100 mg/kg |
| Administration: | I.p. 2 hours after the onset of ischemia |
| Result: |
Reduced the neurological de?cit score.
Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα. Decreased the expressions of ICAM-1, COX-2, and iNOS. Downregulated downstream in?ammatory factor PGE2 and TNF-α expression. Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression. |