| Identification | Back Directory | [Name]
IMR-1 | [CAS]
310456-65-6 | [Synonyms]
IMR-1
IMR-1, >98%
IMR-1 >=98% (HPLC)
2-Methoxy-4-(4-oxo-2-thioxo-thiazolidin-5-ylidenemethyl)-phenoxy]-acetic acid ethyl ester
[2-Methoxy-4-[(4-oxo-2-thioxo-5-thiazolidinylidene)methyl]phenoxy]acetic acid ethyl ester
Acetic acid, 2-[2-methoxy-4-[(4-oxo-2-thioxo-5-thiazolidinylidene)methyl]phenoxy]-, ethyl ester | [Molecular Formula]
C15H15NO5S2 | [MDL Number]
MFCD04141288 | [MOL File]
310456-65-6.mol | [Molecular Weight]
353.41 |
| Chemical Properties | Back Directory | [storage temp. ]
2-8°C(protect from light) | [solubility ]
Soluble in DMSO (up to 45 mg/ml) or in Ethanol (up to 9 mg/ml with warming). | [form ]
solid | [color ]
Yellow | [Stability:]
Stable for 2 years from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20° for up to 1 month. |
| Hazard Information | Back Directory | [Description]
Inhibitor of Mastermind Recruitment-1 (IMR-1, 310456-65-6) disrupts the recruitment of Mastermind-like 1 to the Notch transcriptional activation complex (NTC) on chromatin, which attenuates Notch target gene transcription.1?IC50=26 μM (in vitro assay). IMR-1 inhibits the growth of Notch-dependent cell lines and attenuates the growth of patient-derived tumor xenografts. The ethyl ester is hydrolyzed by intracellular esterases which produces the free acid compound (IMR-1A), IC50=0.5 μM (in vitro?assay). Binding of IMR-1 to NTC is non-covalent and reversible.1 | [in vitro]
in order to determine the effect of imr-1 on the assembly of the notch ternary complex (ntc) in cells, notch-dependent cell lines oe33 and 786-0 were treated with imr-1 or dapt. results showed that treatment of oe33 and 786-0 with imr-1 could decrease the occupancy of maml1 on the hes1 promoter but, in contrast to dapt, imr-1 treatment could not affect the occupancy of notch1 on the hes1 promoter. in addition, western blot analyses indicated that imr-1 treatment did not change the cellular levels of nicd [1]. | [in vivo]
animal study showed that treatment of mice with 15 mg/kg imr-1 could readily block tumor establishment. moreover, imr-1 treatment at 15 mg/kg caused no observable adverse effects on the animal. in two independent pdx models, imr-1 could significantly abrogate the tumor growth to a similar level achieved with dapt treatment, without any significant weight loss or other visible signs of adverse effects in the treated mice [1]. | [IC 50]
26 μmol/l | [References]
1) Austudilo?et al.?(2016),?The Small Molecule IMR-1 Inhibits the Notch Transcriptional Activation Complex to Suppress Tumorigenesis; Cancer Res.,?76?3593 |
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