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ChemicalBook CAS DataBase List L(+)-Lactic acid
79-33-4

L(+)-Lactic acid synthesis

11synthesis methods
-

Yield:17392-83-5 98%

Reaction Conditions:

with marine microbial esterase in aq. phosphate buffer at 37; pH=7.5; for 1 h;Resolution of racemate;Enzymatic reaction;enantioselective reaction;pH-value;Temperature;Solvent;Reagent/catalyst;Time;

Steps:

2.6. Optical resolution of racemic methyl lactate by PHE14

A standard 500-μL hydrolytic reaction system containing140 μg purified esterase PHE14, 50 mmol/L substrate (racemicmethyl lactate) and 50 mmol/L phosphate buffer (pH 7.5) wasincubated at 37 °C for 1 h. After the completion of the enzymaticreaction, reaction samples were extracted with an equalvolume of ethyl acetate and the organic phase was further analyzedto evaluate the enzymatic resolution of (±)-methyl lactate.

References:

Wang, Yilong;Zhang, Yun;Sun, Aijun;Hu, Yunfeng [Cuihua Xuebao/Chinese Journal of Catalysis,2016,vol. 37,# 8,p. 1396 - 1402]

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