D-Phenylalanine synthesis
- Product Name:D-Phenylalanine
- CAS Number:673-06-3
- Molecular formula:C9H11NO2
- Molecular Weight:165.19
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Yield:89 % ee
Reaction Conditions:
with Petroselinum crispum phenylalanine ammonia-lyase-immbolized on tris(4-hydroxyphenyl)methane triglycidyl ether/ethylenediaminetetraacetic dianhydride-functionalized magnetic nanoparticles in aq. buffer at 30; pH=8.8; for 5 h;Enzymatic reaction;stereoselective reaction;
Steps:
3.4. Reusability tests in ammonia elimination reaction of D,L-phenylalanine
General procedure: In 4 mL screw cap glass vials D,L-phenylalanine solution (2 mL, 10 mM D,L-Phe in 100 mM TRIS,pH 8.8) was added to the PcPAL-MNPs-NPDGE/EDa-10 or the PcPAL-MNPs-THPMTGE/EDa-10biocatalysts (15 mg wet biocatalysts) and the resulted suspension was shaken at 600 rpm, 30 °C for 5h. After 5 h samples (50 μL, each) were taken and analyzed by HPLC (for HPLC methods see SISection 2.1). Between the cycles the samples were washed with TRIS buffer (100 mM, pH 8.8) threetimes.
References:
Sánta-Bell, Evelin;Molnár, Zsófia;Varga, Andrea;Nagy, Flóra;Hornyánszky, Gábor;Paizs, Csaba;Balogh-Weiser, Diána;Poppe, László [Molecules,2019,vol. 24,# 22,art. no. 4146] Location in patent:supporting information
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673-06-3
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