ChemicalBook CAS DataBase List 4-Isopropenylphenol

4-Isopropenylphenol synthesis

14synthesis methods

Product Name:4-Isopropenylphenol
CAS Number:4286-23-1
Molecular formula:C9H10O
Molecular Weight:134.18

99-89-8 Synthesis

99-89-8
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$13.00/10g

4286-23-1
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$34.00/100mg

2948-47-2 Synthesis

2948-47-2
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Yield:2948-47-2 56.8 %Chromat. ，4286-23-1 9.7 %Chromat.

Reaction Conditions:

Stage #1:4-Isopropylphenol with 4-methylphenyl phosphate synthase;ATP;magnesium chloride;manganese(ll) chloride in aq. buffer; pH=8 at 30; for 2 h;Enzymatic reaction;
Stage #2: with ferredoxin reductase;ferredoxin;P₄₅₀ monooxygenase;NADPH in aq. buffer; pH=8 at 30; for 2 h;Enzymatic reaction;
Stage #3: with phosphohydrolase in aq. buffer; pH=8 at 30; for 2 h;Enzymatic reaction;

Steps:

Enzymatic Assays
General procedure: Unless specified otherwise, all enzymatic assays were carriedout in 100 μL of 50 mM Tris·HCl buffer (pH 8.0) at 30 °C for 2 h, and thefinal concentration of each enzyme was 10 μM. Methanol (3× volume) wasadded to quench reactions. Precipitated proteins were removed by centrifugationat 20,000 × g for 10 min and the supernatants were used as LC-MSsamples. For reactions catalyzed by CreHI, the reaction mixture contained1 mM substrate, 20 mM MgCl2, 2 mM MnCl2, 2 mM ATP, and purified CreHand CreI. For CreJEF activity toward phosphorylated compounds (2′-9′), theCreHI reactions were used to generate the phosphorylated substrates. Next,CreJ, CreE, and CreF together with 3 mM NADPH were added into the individualpost-CreHI reaction mixture.For one-pot chemomimetic alkylphenol oxidation reactions, the reactionmixtures contained CreHI, CreJEF, and the two optional enzymes CreG (with2 mM NAD+) and CreC (with 2 mM NADP+), 1 mM substrate 2-7, and necessarycofactors, including 20 mM MgCl2, 2 mM MnCl2, 2 mM ATP, and 3 mMNADPH. After incubation at 30 °C for 2 h, CreD was added into each of theone-pot mixtures, followed by another 2-h incubation at 30 °C.

References:

Du, Lei;Dong, Sheng;Zhang, Xingwang;Jiang, Chengying;Chen, Jingfei;Yao, Lishan;Wang, Xiao;Wan, Xiaobo;Liu, Xi;Wangi, Xinquan;Huang, Shaohua;Cui, Qiu;Feng, Yingang;Liu, Shuang-Jiang;Li, Shengying [Proceedings of the National Academy of Sciences of the United States of America,2017,vol. 114,# 26,p. E5129 - E5137]

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80-05-7 Synthesis