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Metabolic Engineering Communications

Metabolic Engineering Communications

IF: 3.7
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Engineering Yarrowia lipolytica for the biosynthesis of geraniol

Published:2 November 2023 DOI: 10.1016/j.mec.2023.e00228
Ayushi Agrawal , Zhiliang Yang , Mark Blenner

Abstract

Geraniol is a monoterpene with wide applications in the food, cosmetics, and pharmaceutical industries. Microbial production has largely used model organisms lacking favorable properties for monoterpene production. In this work, we produced geraniol in metabolically engineered Yarrowia lipolytica. First, two plant-derived geraniol synthases (GES) from Catharanthus roseus (Cr) and Valeriana officinalis (Vo) were tested based on previous reports of activity. Both wild type and truncated mutants of GES (without signal peptide targeting chloroplast) were examined by co-expressing with MVA pathway enzymes tHMG1 and IDI1. Truncated CrGES (tCrGES) produced the most geraniol and thus was used for further experimentation. The initial strain was obtained by overexpression of the truncated HMG1, IDI and tCrGES. The acetyl-CoA precursor pool was enhanced by overexpressing mevalonate pathway genes such as ERG10, HMGS or MVK, PMK. The final strain overexpressing 3 copies of tCrGES and single copies of ERG10, HMGS, tHMG1, IDI produced approximately 1?g/L in shake-flask fermentation. This is the first demonstration of geraniol production in Yarrowia lipolytica and the highest de novo titer reported to date in yeast.

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Related products
Procduct Name CAS Molecular Formula Supplier Price
Geraniol 106-24-1 C10H18O 664 suppliers $6.00-$6476.29
Acetyl coenzyme A sodium salt 102029-73-2 C23H38N7O17P3S.3Na 199 suppliers $33.00-$1490.00
5-MALEIMIDO VALERIC ACID 57078-99-6 C9H11NO4 78 suppliers $95.00-$1680.00
Valerian, ext. 8057-49-6 37 suppliers Inquiry
IDI1 8 suppliers Inquiry
Yarrowia lipolytica 3 suppliers Inquiry
HMGS 1 suppliers Inquiry
AAMPS CATHARANTHUS ROSEUS MONOGRAPH 1 suppliers Inquiry

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