天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

ChemicalBook >> journal list >> Food Chemistry >>article
Food Chemistry

Food Chemistry

IF: 8.5
Download PDF

Immunoassay for the detection of cyproconazole in foods: From hapten synthesis to the establishment of a gold immunochromatographic assay

Published:24 October 2023 DOI: 10.1016/j.foodchem.2023.137847 PMID: 37913707
Yang Liu, Lingling Guo, Liqiang Liu, Xinxin Xu, Hua Kuang, Liguang Xu, Chuanlai Xu

Abstract

Cyproconazole (CPZ) is extensively used in agricultural production. However, its overuse can lead to high residue problems in crops. Existing detection methods are still dominated by instrumental methods and the development of rapid, sensitive field detection remains a challenge. In this study, we designed a novel hapten synthetic pathway and prepared a monoclonal antibody (mAb) that could specifically recognize CPZ with high sensitivity (half inhibition rate was 0.27?ng/mL). From this, a gold immunochromatographic assay (GICA) for the detection of CPZ was established by combining the mAb with gold nanoparticles, with limits of detection in rice, tomatoes and grapes of 0.02?mg/kg, 0.01?mg/kg and 0.05?mg/kg, respectively. The spiked recoveries ranged from 86.5?% to 115.1?%, and the results showed that the GICA was not significantly different from detection using LC-MS/MS. Therefore, we have successfully developed a GICA method for the reliable in situ, rapid and sensitive detection of CPZ.

Substances (3)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Pyrazinecarbonitrile 19847-12-2 C5H3N3 412 suppliers $6.00-$1083.16
GOLD 7440-57-5 Au 288 suppliers $43.30-$9840.00
Cyproconazole 94361-06-5 C15H18ClN3O 202 suppliers $35.00-$1580.00