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Biomedical Chromatography

Biomedical Chromatography

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Absolute oral bioavailability of fenofibric acid and choline fenofibrate in rats determined by ultra-performance liquid chromatography tandem mass spectrometry.

Published:1 April 2017 DOI: 10.1002/bmc.3832 PMID: 27594083
Xudan Wei, Ping Li, Meina Liu, Yuqian Du, Menglin Wang, Jinling Zhang, Jing Wang, Hongzhuo Liu, Xiaohong Liu

Abstract

Choline fenofibrate is the choline salt of fenofibric acid, which releases free fenofibric acid in the gastrointestinal tract. To estimate the absolute oral bioavailability of fenofibric acid and choline fenofibrate, a novel and sensitive UPLC-MS/MS method with liquid-liquid extraction procedure was developed for the determination of fenofibric acid in rat plasma. The separation was achieved on a Phenomenex Kinetex C18 column (50?×?2.1?mm, 2.6?μm) containing 2?mm ammonium acetate-methanol with a gradient elution program. Validations of this method including specificity, sensitivity (limit of quantification, 5?ng/mL), linearity (0.005-10?μg/mL), accuracy (within ±4.3%), precision (intra- and inter-day coefficient of variation <11.3%), recovery (94.9-105.2% for fenofibric acid), matrix effect, stability and dilution, were all within acceptable limits. This method successfully supported the determination of fenofibric acid and choline fenofibrate. The absolute oral bioavailability was 93.4% for choline fenofibrate and 40.0% for fenofibric acid. These results suggested that choline fenofibrate and fenofibric acid had a better in vivo pharmacokinetic behavior than that of fenofibrate. The two new orally administrated pharmaceuticals, fenofibric acid and choline fenofibrate, can be developed as alternatives to fenofibrate.

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Procduct Name CAS Molecular Formula Supplier Price
Fenofibrate 49562-28-9 C20H21ClO4 712 suppliers $5.00-$546.00
Fenofibric acid 42017-89-0 C17H15ClO4 432 suppliers $5.00-$1500.00
Choline Fenofibrate 856676-23-8 C22H28ClNO5 144 suppliers $6.00-$2691.10