天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

How to prepare Tricine buffer

Jul 22,2024

Tricine is an organic compound that is used in buffer solutions. The name tricine comes from tris and glycine, from which it was derived. It is a white crystalline powder that is moderately soluble in water.

Tricine is a commonly used electrophoresis buffer for resuspension of cell pellets. It has a lower negative charge than glycine, allowing it to migrate faster. In addition, its high ionic strength causes more ion movement and less protein movement. This allows low molecular weight proteins to be separated in lower percent acrylamide gels. A discontinuous SDS-PAGE system for the separation of proteins in the range from 1 to 100 kDa was described by Schagger and von Jagow in 1987. Tricine, the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations than in glycine–SDS-PAGE systems. A superior resolution of proteins, especially in the range between 5 and 20 kDa, is achieved without using urea. Proteins above 30 kDa are already destacked within the sample gel. Thus, a smooth passage of these proteins from the sample to the separating gel is warranted, and overloading effects are reduced. This is of special importance when large amounts of protein are to be loaded onto preparative gels. The omission of glycine and urea prevents disturbances that might occur during subsequent amino acid sequencing.

Tricine

Tricine (1 M, pH 8.05) Preparation and Recipe:

Prepare 800 mL of dH2O in a suitable container.

Add 179.17 g of Tricine to the solution.

Adjust the solution to the desired pH with 10N NaOH.

Add dH2O until the volume is 1 L.

  • Related articles
  • Related Qustion
See also
4

L(+)-Lactic acid, also known as milk acid occurs naturally in several foods and is primarily found in fermented milk products, such as: sour milk, cheese, buttermilk and yogurt.....

Jan 24,2025Biochemical Engineering
4

Geraniol is known to be derived from geranyl diphosphate (GPP) by related synthases based on a common ionization-dependent reaction mechanism.....

Jul 22,2024Flavors and fragrances

Tricine

5704-04-1

Tricine manufacturers

  • Tricine
  • 5704-04-1 Tricine
  • $0.00 / 1kg
  • 2025-01-21
  • CAS:5704-04-1
  • Min. Order: 1kg
  • Purity: 98
  • Supply Ability: 1000
  • Tricine
  • 5704-04-1 Tricine
  • $0.00 / 1Kg/Bag
  • 2025-01-20
  • CAS:5704-04-1
  • Min. Order: 1KG
  • Purity: 99%-101%
  • Supply Ability: 5 tons
  • Tricine
  • 5704-04-1 Tricine
  • $1.00 / 1g
  • 2025-01-06
  • CAS:5704-04-1
  • Min. Order: 1g
  • Purity: 99%
  • Supply Ability: 6000