產(chǎn)品英文名稱: Recombinant Human Monocyte Chemotactic Protein-1/MCAF (CCL2)
英文簡(jiǎn)稱:MCP 1
中文名稱:重組人單核細(xì)胞趨化蛋白-1
背景資料:Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B (NF?B). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.
產(chǎn)品描述:人單核細(xì)胞趨化蛋白-1重組蛋白是在大腸桿菌中重組表達(dá)的,是一條非糖基化的多肽鏈,包含有76個(gè)氨基酸,分子量為8607Da.該人單核細(xì)胞趨化蛋白-1重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出.
來(lái)源宿主:大腸桿菌中重組表達(dá)
序列:The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Ile.
溶解建議:提供的人單核細(xì)胞趨化蛋白-1(MCP 1)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末;該凍干粉末是從含1mg/ml人單核細(xì)胞趨化蛋白-1(MCP 1)的溶液(不含添加劑)中凍干產(chǎn)生的.我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到不低于100ug/ml,后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋.
保存建議:人單核細(xì)胞趨化蛋白-1(MCP 1)凍干粉末在室溫可保存3周,或置于-18℃凍存用于長(zhǎng)期保存.重新溶解后可以在4℃冷藏保存至少2-7天;如果想延長(zhǎng)保存時(shí)間,建議分裝(加載體蛋白如0.1% HSA或 BSA)后置于-18℃凍存.請(qǐng)避免反復(fù)凍融.
其他簡(jiǎn)介:? ProSpec是一家全球著名細(xì)胞因子蛋白及相關(guān)抗體生產(chǎn)和研發(fā)品牌。ProSpec的生產(chǎn)和研發(fā)工廠位于以色列,專注于蛋白(重組及合成)生產(chǎn)研發(fā),其獨(dú)有的細(xì)菌和哺乳動(dòng)物表達(dá)和蛋白折疊技術(shù)使其能在17年內(nèi)成長(zhǎng)為國(guó)際一流的科研級(jí)蛋白供應(yīng)商。目前ProSpec可以提供細(xì)胞因子,生長(zhǎng)因子,激素,信號(hào)蛋白,病毒抗原等近800種重組蛋白和100多種抗體,是世界上提供蛋白品種最多的公司之一,領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白,價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白,而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液,ProSpec-Tany絕大部分產(chǎn)品沒(méi)有添加保護(hù)劑或鹽,這就對(duì)工藝提出了更高的要求。同時(shí),ProSpec-Tany絕大部分產(chǎn)品為凍干粉,因此易于運(yùn)輸和保存。
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