Amonafide

價格	￥977.75
包裝	1EA

最小起訂量	1EA
發(fā)貨地	其他
更新日期	2020-01-12

產(chǎn)品詳情

英文名稱:Amonafide	CAS:69408-81-7
規(guī)格: 10mg/10mM/1mL/50mg/100mg	貨號: S1367

Selleck Chemicals美國品牌，中國庫存現(xiàn)貨，Topoisomerase抑制劑，CAS#69408-81-7。
更多詳情請訪問中國唯一官方網(wǎng)站： http://www.selleck.cn/products/Amonafide.html

生物活性

產(chǎn)品描述	Amonafide (NSC-308847)通過topoisomerase II（拓撲異構(gòu)酶II）介導(dǎo)導(dǎo)致蛋白相關(guān)的DNA鏈斷裂，但不會產(chǎn)生拓撲異構(gòu)酶I介導(dǎo)的DNA裂解。Phase 3。
靶點	Topoisomerase II	?	?	?	?	?
IC50	?	?	?	?	?	?
體外研究	Through a topoisomerase II-mediated reaction, Amonafide treatment produces DNA single-strand breaks (SSB), double-strand breaks (DSB), and DNA-protein cross-links in human myeloid leukemia cells. Amonafide treatment inhibits conlony formation of the leukemic cell lines and the normal human bone marrow GM-CFC in a dose-dependent manner. Amonafide does not produce topoisomerase I-mediated DNA cleavage even at 100 μM. The m-AMSA-resistant line is less than 2-fold resistant to Amonafide? [1] ?Amonafide interferes with the DNA breakage-reunion activity of mammalian DNA topoisomerase II resulting in DNA cleavage stimulation.? [2] ?Compared with those of other antitumor drugs, Amonafide-stimulated cleavage intensity patterns are markedly different. Amonafide highly prefers a cytosine, and excludes guanines and thymines instead, at position -1, with lower preference for an adenine at position +1.? [3] ?Topoisomerase II-mediated DNA cleavage induced by Amonafide is affected only slightly (less than 3-fold) by 1 mM ATP, suggeting that Amonafide is an ATP-insensitive topoisomerase II inhibitor in contrast to doxorubicin, etoposide, and mitoxantrone.? [4] ?Amonafide significantly inhibits the growth of HT-29, HeLa, and PC3 cells with IC50 of 4.67 μM, 2.73 μM, and 6.38 μM, respectively.? [5] ?Amonafide is unaffected by P-glycoprotein-mediated efflux, unlike those of the classical topoisomerase II inhibitors (daunorubicin, doxorubicin, idarubicin, etoposide, and mitoxantrone).? [6]
體內(nèi)研究	?
臨床實驗	A Phase I/II study of Amonafide in men with androgen-independent prostate cancer has been completed.
特征	?

細胞試驗:?
[5]

細胞系	HT-29, HeLa, and PC3
濃度	Dissolved in DMSO, final concentrations ~10 μM
處理時間	72 hours
方法	All cell lines are in the logarithmic phase of growth when the assay of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is carried out. Cells are harvested and seeded into 96-well tissue culture plates at a density of 2.5 × 103?cells/well in 150 μL aliquots of medium. The concentrations tested are serial dilutions of a stock solution (10 μM in DMSO) with phosphate-buffered saline (PBS) and are added 24 hours later. The assay is ended after 72 hours of Amonafide exposure and PBS is used as a negative control. After 72 hours treatment, cells are washed twice with PBS, and then 50 μL/well of MTT reagent (1 mg/mL in PBS) together with 150 μL/well of prewarmed medium are added. The plates are returned to the incubator for 4 hours. Subsequently, DMSO is added as solvent. Absorbance is determined at 570 nm with a Microplate reader. All experiments are performed at least three times, and the average of the percentage absorbance is plotted against concentration. Then, the concentration of Amonafide required to inhibit 50% of cell growth (IC50) is calculated for Amonafide.

溫馨提示：不可用于臨床治療。

關(guān)鍵字： Amonafide;化學試劑

公司簡介

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Amonafide相關(guān)廠家報價

產(chǎn)品名稱	價格		公司名稱	報價日期
5-氨基-2-(2-(二甲基氨基)乙基)-1H-苯并[de]異喹啉-1,3(2H)-二酮	詢價	VIP5年	河南威梯?；た萍加邢薰?/div>	2024-12-24
氨萘非特	詢價	VIP5年	陜西締都醫(yī)藥化工有限公司	2024-12-18
氨萘非特\|T6336\|TargetMol	￥218	VIP3年	TargetMol中國（陶術(shù)生物）	2024-12-12
Amonafide	￥1600		上海超研生物科技有限公司	2024-12-08
aladdin 阿拉丁 A408632 Amonafide 69408-81-7 10mM in DMSO	￥451.90	VIP1年	上海阿拉丁生化科技股份有限公司	2024-11-29
aladdin 阿拉丁 A126932 AS1413, 氨萘非特(Amonafide) 69408-81-7 ≥98%	￥338.90	VIP13年	上海阿拉丁生化科技股份有限公司	2024-10-31
Amonafide (AS1413)	￥900		南京百鑫德諾生物科技有限公司	2024-09-26
Amonafide	￥875		武漢科昊佳生物科技有限公司	2020-01-13
Amonafide	￥700		上海偉寰生物科技有限公司	2020-01-13

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