稱 Tris-Base（三羥甲基氨基甲烷）

規(guī)格 100g 

保存 常溫保存，長(zhǎng)期有效

產(chǎn)品簡(jiǎn)介 

Tris base，即Tris堿，中文名也稱為三(羥甲基)氨基甲烷，即生物化學(xué)和分子生物學(xué)中常常被配制成多種緩沖液，例如：Tris-EDTA（TE緩沖液），多用于核酸的純化和儲(chǔ)存，保護(hù)其不被核酸酶降解；Tris acetate-EDTA（TAE緩沖液）和Tris borate-EDTA（TBE緩沖液）普遍用作凝膠配制緩沖液以及電泳緩沖液。

Tris緩沖液不僅被廣泛用作核酸和蛋白質(zhì)的溶劑，還有許多重要用途。Tris被用于不同pH條件下的蛋白質(zhì)晶體生長(zhǎng)。Tris緩沖液的低離子強(qiáng)度特點(diǎn)可用于線蟲(chóng)（C. elegans）核纖層蛋白（lamin）的中間纖維的形成。Tris也是蛋白質(zhì)電泳緩沖液的主要成分之一。此外，Tris還是制備表面活性劑、硫化促進(jìn)劑和一些藥物的中間物。Tris也被用作滴定標(biāo)準(zhǔn)物。

緩沖特性

Tris為弱堿，在室溫（25℃）下，它的pKa為8.1；根據(jù)緩沖理論，Tris緩沖液的有效緩沖范圍在pH7.0到9.2之間。

Tris堿的水溶液pH在10.5左右，一般加入鹽酸以調(diào)節(jié)pH值至所需值，即可獲得該pH值的緩沖液。但同時(shí)應(yīng)注意溫度對(duì)于Tris的pKa的影響。

產(chǎn)品性質(zhì) 

中文別名（Chinese synonym） 2-氨基-2-(羥甲基)-1,3-丙二醇；Tris 堿；氨基丁三醇；三(羥甲基)氨基甲烷；
英文別名（English synonym） THAM; Trizma base; Trometamol; Tris(hydroxymethyl)aminomethane;
2-Amino-2-(hydroxymethyl)-1,3-propanediol;
CAS號(hào)（CAS NO.） 77-86-1
分子式（Formula） NH2C(CH2OH)3
分子量（Molecular weight） 121.14
外觀（Appearance） 白色結(jié)晶粉末至針狀結(jié)晶
熔點(diǎn)（Melting point） 168-172 °C
溶解性（Solubility） 溶于水

注意事項(xiàng) 

為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids.

Buffering features
The conjugate acid of Tris has a pKa of 8.07 at 25 °C, which implies that the buffer has an effective pH range between 7.1 and 9.1 (pKa ± 1) at room temperature.

Buffer details
The pKa declines approximately 0.03 units per degree Celsius rise in temperature.
Silver-containing single-junction pH electrodes (e.g., silver chloride electrodes) are incompatible with tris since an Ag-tris precipitate forms which clogs the junction. Double-junction electrodes are resistant to this problem, and non-silver containing electrodes are immune.

Other names
TRIS, Tris, Tris base, Tris buffer, Trizma, Trisamine, THAM, Tromethamine, Trometamol, Tromethane, Trisaminol