???????Choline Kinase，中文名為膽堿激酶，以三磷酸腺苷(ATP)作為磷酸的供體，催化膽堿發(fā)生磷酸化。本產(chǎn)品為大腸桿菌表達的膽堿激酶，并經(jīng)簡單純化獲得的可以確保達到一定酶活力的粗酶液。
???????由于本產(chǎn)品需新鮮制備，確認(rèn)訂購后約6個工作日才可以發(fā)貨。個別情況首次制備出現(xiàn)問題，發(fā)貨時間會順延。
???????本產(chǎn)品可以提供十公斤、百公斤乃至噸級的粗酶液，如有需求請聯(lián)系碧云天。
???????Choline kinase (also known as CK,ChoK and choline phosphokinase) is an enzyme which catalyzes the first
reaction in the choline pathway for phosphatidylcholine (PC) biosynthesis. This reaction involves the transfer of a phosphate group from adenosine triphosphate (ATP) to choline in order to form phosphocholine. Thus, the two substrates of this enzyme are ATP and choline, whereas its two products are adenosine diphosphate (ADP) and O-phosphocholine. Choline kinase requires magnesium ions (+2) as a cofactor for this reaction. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The first detailed investigation of the enzyme was conducted by McCamen in 1962, where it was shown that the brain is the richest source of the enzyme in mammalian tissue. A related enzyme, ethanolamine kinase, tends to co-purify with choline kinase leading to a suggestion that the two activities are mediated by two distinct active sites on a single protein. These enzymes participate in glycine, serine and threonine metabolism and glycerophospholipid metabolism. In mammalian cells, the enzyme exists as three isoforms: CKα-1,CKα-2 and CKβ. These isoforms are encoded by two separate genes, CHKA and CHKB and are only active in their homodimeric, heterodimeric and oligomeric forms. This product with the indicated enzyme activity was briefly purified from engineered E.coli.
產(chǎn)品詳細信息見下表:

包裝清單：

保存條件：
???????-20℃或更低溫度保存，至少1個月有效。
注意事項：
???????由于本產(chǎn)品需新鮮制備，在您確認(rèn)訂購后約6個工作日才能發(fā)貨。個別情況首次制備出現(xiàn)問題，發(fā)貨時間會順延。
???????粗酶液的每次凍融均可能會引起部分失活，所以收到酶液后請根據(jù)需要進行分裝，并置于-20℃或更低溫度保存。
???????隨著保存時間的延長，酶活力會有一定程度的下降，收到產(chǎn)品后應(yīng)盡快使用。
???????本產(chǎn)品在生產(chǎn)和保存的過程中可能會有混濁或沉淀產(chǎn)生，融化后混勻即可，不影響正常使用。
???????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
???????為了您的安全和健康，請穿實驗服并戴一次性手套操作。