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PureLink Pro 96 Viral RNA/DNA Purification Kit
  • PureLink Pro 96 Viral RNA/DNA Purification Kit

PureLink Pro 96 Viral RNA/DNA Purification Kit

價格 16269
包裝 4plates
最小起訂量 4plates
發(fā)貨地 上海
更新日期 2024-12-08

產(chǎn)品詳情

英文名稱:PureLink Pro 96 Viral RNA/DNA Purification Kit產(chǎn)品類別: DNA提取與純化
2024-12-08 PureLink Pro 96 Viral RNA/DNA Purification Kit PureLink Pro 96 Viral RNA/DNA Purification Kit 4plates/16269RMB 16269 DNA提取與純化

描述

The PureLink® Pro 96 Viral RNA⁄DNA Purification Kit is the only 96-well silica plate system that can extract both viral RNA and DNA from 200-µl cell-free fluids which uses a low-depth 96-well centrifuge bucket for ease-of- use, all without any loss in sensitivity. These kits provide:
• Versatility-one kit for all your samples, the protocol is optimized for both viral RNA and DNA extraction
• Flexibility in centrifuge choice-minimum speeds for 96 well processing of 2250 x g and a rotor bucket depth of 5 cm make this kit one of the most adaptable systems for existing laboratory centrifuges (Figure 1)
• Sensitivity and reproducibility as good as a spin column-moving to high throughput is reliable and easy with no loss in accuracy of quantitation

The PureLink® Pro 96 Viral RNA⁄DNA Purification Kit is based on conventional silica plate extraction chemistry. Cell-free samples are lysed in guanidine containing buffers that have been reformulated to increase the recovery and sensitivity of purified nucleic acids, while carrier RNA is used to protect samples from RNase degradation. Binding of lysates to the Viral Filter Plate can be performed with either a vacuum manifold or centrifugation using a 96-well rotor with buckets having a depth of only 5 cm. After washes with ethanol-based buffers, the viral RNA or DNA is eluted in 100 µl of RNase-free water. The RNA or DNA is ready to use in one-step or two-step RT-PCR and qRT-PCR, qPCR, or other enzymatic amplification procedures.

The PureLink® Pro 96 Viral RNA⁄DNA Purification Kit shows excellent well-to-well consistency
A critical requirement for any 96-well purification system is well-to-well consistency. When using a technique as sensitive as qRT-PCR for viral nucleic acid detection, which may exist as low viral titers from patient samples, consistency between samples becomes even more important. When well-to-well recovery of lentiviral RNA was analyzed using the PureLink® Pro 96 Viral RNA⁄DNA Purification Kit, consistent Ct values were obtained in qRT-PCR analyses while plasma controls run on the same plate were negative, showing no cross contamination (Figure 2).

Get the same results in a 96-plate extraction as you would with a spin column
To prove consistency of our novel purification chemistry in the both high- and low-throughout formats, Armored RNA® HCV virus was spiked in human plasma and 200 µl were used for viral nucleic acid purification with either the PureLink® Viral RNA⁄DNA Mini Kit (spin-column based purification) or with the PureLink® Pro 96 Viral RNA⁄DNA Purification Kit. The purified samples were analyzed for performance comparison using qRT-PCR. Both purification methods successfully demonstrated viral purification and detection as both purification profiles exhibited scalable qRT-PCR detection analysis profiles (Figure 3). The PureLink® Pro 96 Viral RNA⁄DNA Purification Kit provides sensitive linear scalable viral RNA⁄DNA purification with a sensitivity and efficiency equal to that of the spin column purification format.

關(guān)鍵字: PureLink Pro 96 Viral RNA/DNA Purification Kit

公司簡介

Thermo Fisher Scientific is the world leader in serving science, with revenues of more than $24 billion and approximately 70,000 employees globally. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, deliver medicines to market and increase laboratory productivity. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – we offer an unmatched combination of innovative te
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  • 公司成立:22年
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  • 企業(yè)類型:有限責任公司(臺港澳法人獨資)
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詢盤

PureLink Pro 96 Viral RNA/DNA Purification Kit相關(guān)廠家報價

產(chǎn)品名稱 價格   公司名稱 報價日期
¥1190
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