95%的大腸桿菌具有β-葡萄糖苷酶基因,用這種發(fā)色底物的培養(yǎng)基可以檢測(cè)以及定量食品樣本如肉、奶制品以及貝類中的大腸桿菌數(shù)量,以及在臨床上檢測(cè)尿路感染。國(guó)際上普遍采用該產(chǎn)品取代傳統(tǒng)方法以精確檢測(cè)飲用水中的大腸桿菌數(shù)量,該方法大大降低了假陽(yáng)性和假陰性。該產(chǎn)品也用于快速檢測(cè)植物中GUS基因融合標(biāo)記。β-葡糖醛酸酶(β-glucuronidase (GUS))基因檢測(cè)的顯色底物。
X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.Chromogenic substrate for β-glucuronidase (GUS) gene detection. Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
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