Human KIR2DL5 / CD158f Protein, Fc Tag

分子別名（Synonym）

KIR2DL5,CD158f

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human KIR2DL5 / CD158f Protein, Fc Tag (KIA-H5255) is expressed from human 293 cells (HEK293). It contains AA His 22 - His 240 (Accession # Q8N109-1).

Predicted N-terminus: His 22

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 50 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine,25 mM Arg,150 mM NaCl,pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

殺傷細(xì)胞免疫球蛋白樣受體2DL5（KIR2DL5）屬于免疫球蛋白超家族，是NK細(xì)胞的抑制性受體。KIR2DL5由兩個(gè)同源基因編碼，這兩個(gè)基因顯示拷貝數(shù)變異和等位基因多態(tài)性——KIR2DL5A和KIR2DL5B，其結(jié)構(gòu)域僅變化2個(gè)氨基酸，信號(hào)肽僅變化1個(gè)氨基酸。KIR2DL5和KIR2DL4形成一個(gè)亞家族，其細(xì)胞質(zhì)尾部比其他KIR更長(zhǎng)，每個(gè)都有一個(gè)D0和一個(gè)D2型Ig樣結(jié)構(gòu)域。