Human FGF-17 Protein, His Tag (HPLC verified)

分子別名（Synonym）

FGF-17,Fibroblast growth factor 17,FGF17

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human FGF-17 Protein, His Tag (FG7-H5144) is expressed from E. coli cells. It contains AA Thr 23 - Thr 216 (Accession # O60258-1).

Predicted N-terminus: Met

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 24.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

宿主蛋白殘留（Host Cell Protein）

<0.5 ng/μg of protein tested by ELISA.

宿主核酸殘留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

無(wú)菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

成纖維細(xì)胞生長(zhǎng)因子（FGF）是一個(gè)結(jié)構(gòu)相關(guān)的蛋白質(zhì)大家族，參與多種細(xì)胞過(guò)程，包括增殖、分化、遷移和凋亡。FGF17，也稱(chēng)為FGF-13，在胚胎發(fā)生過(guò)程中以及在成年小腦和皮質(zhì)中表達(dá)，可能對(duì)血管生長(zhǎng)和正常大腦發(fā)育至關(guān)重要。此外，FGF17和FGF8是小腦發(fā)育各個(gè)階段中后腦區(qū)域模式化的關(guān)鍵因素，具有復(fù)雜的時(shí)空基因表達(dá)圖。