Frizzled-2產(chǎn)品信息

英文名稱(chēng)：Frizzled-2

中文名稱(chēng)：卷曲蛋白-2

靶點(diǎn)別稱(chēng)：FZD2

物種：Human

標(biāo)簽：Fc Tag

屬性：Protein

標(biāo)記：Unconjugated

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Frizzled-2, Fc Tag (FZ2-H5251) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ser 156 (Accession # AAI13403).

Predicted N-terminus: Gln 24

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 41.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Frizzled-2（FZD2）也被稱(chēng)為FzE2，屬于G蛋白偶聯(lián)受體Fz/Smo家族。大多數(shù)卷曲受體與β-連環(huán)蛋白規(guī)范信號(hào)通路偶聯(lián)，導(dǎo)致凌亂蛋白的激活、GSK-3激酶的抑制、β-連環(huán)素的核積累和Wnt靶基因的激活。FZD2包含一個(gè)FZ（卷曲）結(jié)構(gòu)域。FZD2可能參與組織形態(tài)發(fā)生和/或分化組織中極性信息的轉(zhuǎn)導(dǎo)和細(xì)胞間傳遞。FZD2的Lys-Thr-X-X-X-Trp基序與Dvl（發(fā)育不良）家族成員的PDZ域相互作用，并參與Wnt/β-catenin信號(hào)通路的激活。