| 名稱 | U0126-EtOH |
| 描述 | U0126-EtOH (U0126 Ethanol) is a non-ATP competitive specific inhibitor of MEK1/2 (IC50: 0.07/0.06 μM). |
| 細(xì)胞實驗 | HEK293 cells were maintained in Dulbecco's modification of Eagle's medium (low glucose) plus 10% foetal bovine serum. HeLa cells stably expressing wild type or kinase-dead LKB1 have been described. AMPK activity was determined by immunoprecipitate kinase assays using anti-AMPK-a1 and -a2 antibodies. Antibodies recognising AMPK phosphorylated on Thr-172 (anti-pT172), AMPK-α1 and -α2 and acetyl-CoA carboxylase-1 (ACC1) phosphorylated on Ser-80 [16] were described previously. Quantification of ratios of signals from phosphorylated and total protein using these antibodies was performed by dual labelling using the LI-COR Odyssey IR imager as described. Contents of ATP and ADP were determined for cells in 6 cm culture dishes by quickly pouring off the medium, adding 350 μl of ice-cold 5% perchloric acid, scraping the cells off with a plastic scraper, and centrifuging (14 000 · g; 3 min, 4 °C) to remove insoluble material. The perchloric acid was then extracted from the supernatant and nucleotides analysed by capillary electrophoresis of perchloric acid extracts as described previously. All incubations of cells were performed in triplicate and results are expressed as means ± S.E.M [3]. |
| 激酶實驗 | The amount of immunoprecipitated wild type MEK used in these assays was adjusted to give a similar amount of activity units as obtained with 10 nM recombinant MEK. All other assays were performed with a recombinant, constitutively activated mutant MEK-1 (ΔN3-S218E/S222D) or constitutively active MEK-2(S222E/S226D). Reaction velocities were measured using a 96-well nitrocellulose filter apparatus as described below. Unless otherwise noted, reactions were carried out at an enzyme concentration of 10 nM, in 20 mM Hepes, 10 mM MgCl2, 5 mM β-mercaptoethanol, 0.1 mg/ml BSA, pH 7.4, at room temperature. Reactions were initiated by the addition of [γ-33P]ATP into the premixed MEK/ERK/inhibitor reaction mixture, and an aliquot of 100 μl was taken every 6 min and transferred to the 96-well nitrocellulose membrane plate which had 50 mM EDTA to stop the reaction. The membrane plate was drawn and washed 4 times with buffer under vacuum. Wells were then filled with 30 μl of Microscint-20 scintillation fluid, and the radioactivity of33P-phosphorylated ERK was counted with a Top Count scintillation counter. Velocities were obtained from the slopes of radioactivity versus time plots. Concentrations of ERK and ATP were 400 nM and 40 μM, respectively, unless otherwise indicated [2]. |
| 動物實驗 | Prior to injection, FI cells were labeled with a stable fluorescent dye molecule, DiA at 10 μg/ml for 5 h at 37 1C. After washing to remove free DiA, cells were trypsinized for inoculation (U0126 experiments) or transfection (RNAi experiments). Biliary epithelial cells were injected subcutaneously, at the indicated times, into the tibia of nude mice. In the chemical experiments, 3h after inoculation, mice were treated with U0126 (10.5 mg/kg) daily by intraperitoneal injection. The length and width of each tumor were measured every day by using a caliper. The following formula was used to calculate tumor volumes ? width2 length/2. Mice were killed at the end of experiment. Tumors were immediately frozen in liquid nitrogen [5]. |
| 體外活性 | U0126通過非競爭性抑制具有雙特異性的激酶MEK的活性，從而抵抗AP-1轉(zhuǎn)錄活性，其IC50分別對MEK 1為0.07 microM，對MEK 2為0.06 microM [1]。在用TPA/血清處理的成纖維細(xì)胞中，U0126可以降低c-Fos和c-Jun蛋白的上調(diào)50-80%。使用10 μM U0126處理不會影響SP-1、JunD及Fra-1等本質(zhì)上表達(dá)的轉(zhuǎn)錄因子的蛋白水平[2]。在HEK293細(xì)胞中，U0126導(dǎo)致AMPK的磷酸化和激活，并增加了其下游靶標(biāo)乙酰-CoA羧化酶的磷酸化，這一效應(yīng)僅在表達(dá)上游激酶LKB1的細(xì)胞中發(fā)生[3]。 |
| 體內(nèi)活性 | 將小鼠通過氣霧劑途徑處理U0126，導(dǎo)致了以下結(jié)果：(i) 抑制了肺部MEK激活 (ii) 相比未經(jīng)處理的對照組，減少了后代IAV滴度 (iii) 保護(hù)了IAV感染的小鼠，對抗100倍致死性病毒挑戰(zhàn)[4]。在所有U0126 (10.5 mg/kg) 實驗中，移植和早期腫瘤生長顯著減少。此外，在注射后第9天及以后，用U0126處理的腫瘤體積減少了60-70%。U0126處理的小鼠中Cdk1表達(dá)也大幅降低[5]。 |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 7.9 mg/mL (18.52 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 55 mg/mL (128.93 mM)
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| 關(guān)鍵字 | Influenza Virus | Mitogen-activated protein kinase kinase | MAP2K | U-0126-EtOH | MEK | inhibit | competitive | virus | Mitophagy | Autophagy | U0126EtOH | MAPKK | Mitochondrial Autophagy | U 0126 | Inhibitor | progeny | non-ATP | U0126-EtOH | U0126 EtOH | U-0126 |
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