Biotinylated Mouse PCSK9 Protein, Avitag?,His Tag
分子別名（Synonym）
PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
表達區(qū)間及表達系統(tǒng)（Source）
MABSol? Biotinylated Mouse PCSK9, Avitag,His Tag (PC9-M82E1) is expressed from human HEK293 cells. It contains AA Gln 35 - Gln 694 (Accession # AAH38085.1).
Predicted N-terminus: Gln 35
蛋白結構（Molecular Characterization）
This protein carries an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The protein has a calculated MW of 13.9 kDa and 61.1 kDa. The protein migrates as 16 kDa and 66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
標記（Labeling）
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標記度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
內毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
前蛋白轉化酶枯草桿菌蛋白酶/kexin 9型（PCSK9）也稱為NARC1（神經(jīng)凋亡調節(jié)轉化酶），是一種新鑒定的屬于肽酶S8亞家族的枯草蛋白酶。小鼠PCSK9被合成為可溶性酶原，并在內質網(wǎng)中經(jīng)歷自催化分子內加工，導致其前肽被切割，該前肽仍與分泌的活性酶相關，具有廣泛的堿性pH最佳值。這種蛋白質在膽固醇穩(wěn)態(tài)中起著重要的調節(jié)作用。PCSK9與低密度脂蛋白受體（LDLR）的表皮生長因子樣重復序列A（EGF-A）結構域結合，誘導LDLR降解。PCSK9也可能在皮質神經(jīng)元的分化中發(fā)揮作用。該基因的突變與一種罕見的常染色體顯性家族性高膽固醇血癥（HCHOLA3）有關。
關鍵字： PCSK9;PCSK9蛋白;PCSK9重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領域提供關鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。