Biotinylated Human PCSK9 Protein, Avitag?,His Tag (MALS verified)
分子別名（Synonym）
PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
表達區(qū)間及表達系統(tǒng)（Source）
Biotinylated Human PCSK9, Avitag,His Tag (PC9-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).
Predicted N-terminus: Gln 31
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non covalent interactions and with a calculated MW of 13.8 kDa and 59.9 kDa respectively. The protein migrates as 17 kDa and 63-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
標(biāo)記（Labeling）
Biotinylation of this product is performed using Avitag? technology.
Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標(biāo)記度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>90% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
前蛋白轉(zhuǎn)化酶枯草桿菌蛋白酶/kexin 9型（PCSK9）也稱為NARC1（神經(jīng)細(xì)胞凋亡調(diào)節(jié)轉(zhuǎn)化酶），是一種新發(fā)現(xiàn)的屬于肽酶S8亞家族的枯草酶。小鼠PCSK9被合成為可溶性酶原，并在內(nèi)質(zhì)網(wǎng)中進行自催化分子內(nèi)處理，導(dǎo)致其前肽的切割，該前肽與分泌的活性酶保持關(guān)聯(lián)，具有寬的堿性最佳pH值。這種蛋白質(zhì)在膽固醇穩(wěn)態(tài)中起著重要的調(diào)節(jié)作用。PCSK9與低密度脂蛋白受體（LDLR）的表皮生長因子樣重復(fù)序列A（EGF-A）結(jié)構(gòu)域結(jié)合，誘導(dǎo)LDLR降解。PCSK9也可能在皮層神經(jīng)元的分化中發(fā)揮作用。該基因的突變與一種罕見的常染色體顯性家族性高膽固醇血癥（HCHOLA3）有關(guān)。
關(guān)鍵字： PCSK9;PCSK9蛋白;PCSK9重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。