Mouse IL-33 Protein, His Tag

分子別名（Synonym）

IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse IL-33, His Tag (IL3-M5245) is expressed from human 293 cells (HEK293). It contains AA Ser 109 - Ile 266 (Accession # Q8BVZ5-1).

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.5 kDa. The protein migrates as 20-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素33（IL33）被稱為C9orf26、DKFZp586H0523、DVS27、NF-HEV、NFEHEV、RP11-575C20.2，是一種屬于IL-1超家族的細(xì)胞因子。IL-33誘導(dǎo)輔助性T細(xì)胞、肥大細(xì)胞、嗜酸性粒細(xì)胞和嗜堿性粒細(xì)胞產(chǎn)生2型細(xì)胞因子。IL-33通過與受體ST2（又名IL1RL1）和IL-1受體輔助蛋白（IL1RAP）相互作用，激活NF-κB和MAP激酶信號通路中的細(xì)胞內(nèi)分子，驅(qū)動極化Th2細(xì)胞產(chǎn)生2型細(xì)胞因子（如IL-5和IL-13），從而介導(dǎo)其生物學(xué)效應(yīng)。在體內(nèi)，IL-33誘導(dǎo)IL-4、IL-5和IL-13的表達(dá)，并導(dǎo)致粘膜器官的嚴(yán)重病理變化。