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hygiena 牛奶中酪蛋白/β-乳球蛋白過敏原檢測試劑盒,AlerTox? ELISA Milk (Casein & Beta-lactoglobulin)
  • hygiena 牛奶中酪蛋白/β-乳球蛋白過敏原檢測試劑盒,AlerTox? ELISA Milk (Casein & Beta-lactoglobulin)

hygiena 牛奶中酪蛋白/β-乳球蛋白過敏原檢測試劑盒

價格 詢價
包裝 1盒
最小起訂量 1盒
發(fā)貨地 河南
更新日期 2024-10-12

產(chǎn)品詳情

中文名稱:hygiena 牛奶中酪蛋白/β-乳球蛋白過敏原檢測試劑盒英文名稱:AlerTox? ELISA Milk (Casein & Beta-lactoglobulin)
品牌: Hygiena產(chǎn)地: 美國
保存條件: 2-8℃產(chǎn)品類別: 食品檢測試劑盒 過敏原檢測
數(shù)量: 1保質(zhì)期: 13個月
樣本: 食物,飲料應(yīng)用: 定量檢測原材料和最終產(chǎn)品中的酪蛋白/β-乳球蛋白
檢測方法: 酪蛋白/β-乳球蛋白檢測限: 0.05ppm
貨號: KIT3041用途范圍: 定量檢測原材料和最終產(chǎn)品中的酪蛋白/β-乳球蛋白
規(guī)格: 96T/盒是否進口:
2024-10-12 hygiena 牛奶中酪蛋白/β-乳球蛋白過敏原檢測試劑盒 AlerTox? ELISA Milk (Casein & Beta-lactoglobulin) 1盒/RMB Hygiena 美國 2-8℃ 食品檢測試劑盒 過敏原檢測

hygiena 牛奶中酪蛋白/β-乳球蛋白過敏原檢測試劑盒

AlerTox?ELISA Milk (Casein & Beta-lactoglobulin)



1. Test principle


All AlerTox ELISA tests work on the principle of a quantitative sandwich ELISA. An antibody directed against the target antigen (protein) is bound on the surface of a microtiter plate. Antigen-containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature (15 - 25 oC/59 - 77 oF), the wells are washed with diluted washing solution to remove unbound material. A peroxidase-conjugated second antibody directed against the same antigen is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a Stop Solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of the antigen is directly proportional to the color intensity of the test sample


2. Supplied Materials


1)Breakable strips of 8 wells each coated with antigen-specific antibodies:12 strips


2)5 AlerTox Standards. Ready to use.:5 x 3 mL


2a) 5 AlerTox Standards; 100x concentrated (only AlerTox ELISA Casein & AlerTox ELISA Milk):5 x 1 mL


3) Conjugate solution. Ready to use:1 x 15 mL


4) Substrate solution (TMB). Ready to use:1 x 15 mL


5) Stop Solution, containing H2 SO4 . Ready to use: 1 x 15 mL


6) Extraction & Sample Dilution Buffer 10x:4 x 30 mL


6a) Extraction & Sample Dilution Buffer 5x (only AlerTox ELISA Casein & AlerTox ELISA Milk): 4 x 60 mL


7) Washing Solution 10x:2 x 60 mL


3. Storage advice


? All kit components should be kept at 2 – 8 °C (36 - 46 °F) in the dark. DO NOT FREEZE.


? Return all reagents to 2 – 8 °C (36 - 46 °F) immediately after use.


? The diluted Washing Solution concentrate can be used for 4 weeks, when stored at 2 – 8 °C (36 - 46 °F).


? The diluted Extraction & Sample Dilution Buffer can be used for 1 week, when stored at 2 – 8 °C (36 - 46 °F). When crystals precipitate while storing, warm up to 37 °C (99 °F) for 15 min. before use.


? The Sample Extracts are stable for at least 24 hours at 2 – 8 °C (36 - 46 °F), or frozen for longer storage.


4. Sample preparation


a) Wine samples:


1. Add 0.5 ml of wine to 9.5 ml of extraction buffer 1x and mix.


2. Use directly 100 μl in the ELISA plate. Centrifugation is normally not necessary as long as the mixture is clear. If not, centrifuge as described under b) point 4. For other liquid samples proceed with b) point 3.


b) Other kinds of samples:


1. To maximize homogeneity and representativeness of the sample drawing, a minimum of 5 g sample should be pulverized finely in a mortar, impact mill or a similar device.


2. 0.5 g of the homogenized mixture is suspended in 10 ml of extraction buffer 1x.


3. Incubate the mixture for 15 min in a pre-heated water bath at 60 °C (140 °F) (AlerTox ELISA Crustacean 40 °C (104 °F)). To ensure good homogeneity, the samples should be shaken every two minutes.


4. Centrifuge the mixtures for 10 minutes at 2000 x g. If it is not possible to separate the supernatant from the precipitate completely, then filter the supernatant after centrifugation. Cool down the filtrate to room temperature (15 - 25 oC/59 - 77 oF). (For AlerTox ELISA Casein and AlerTox ELISA Milk kits see also special hints on point 9. Special Hints).


5. 100 μl of this solution are applied per well. If the results of a sample are out of the measuring range, further dilution with the pre-diluted Extraction and Sample dilution buffer is necessary. The additional dilution must be considered when calculating the concentration.


關(guān)鍵字: 牛奶過敏;酪蛋白過敏檢測;β-乳球蛋白檢測;β-乳球蛋白過敏原;

公司簡介

河南歐諾生物科技有限公司是一家產(chǎn)學(xué)研相結(jié)合、科工貿(mào)一體化的,集食品安全及動物防疫檢驗試劑的研發(fā)、生產(chǎn)、銷售及技術(shù)服務(wù)為一體的中國生物高科技企業(yè)。公司作為一家專門從事經(jīng)營食品安全檢測ELISA試劑盒的專業(yè)化公司,自公司成立以來,憑借優(yōu)質(zhì)的產(chǎn)品,合理的價格,完善的服務(wù),以及良好的信譽,得到了廣大客戶的認(rèn)可和支持。? 歐諾OUNUO生產(chǎn)及代理用于檢測獸藥殘留類、霉菌毒素類、維生素類、海藻毒素類、激素類、工業(yè)污染物類、農(nóng)藥殘留類等酶聯(lián)免疫試劑盒,膠體金試紙條。旨在有效保證實驗室產(chǎn)品質(zhì)量,為客戶提供優(yōu)質(zhì)服務(wù)。我司現(xiàn)已成為:美國ABRaxis、美國HELICA,美國BIOO,美國BIOSTONE,英國朗道等進口品牌試劑盒特約優(yōu)秀代理商。
成立日期 2022-08-22 (3年) 注冊資本 200萬人民幣
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 生化試劑,診斷試劑,農(nóng)業(yè)與食品分析儀器,色譜柱 經(jīng)營模式 工廠,試劑,定制
  • 河南歐諾生物科技有限公司
非會員
  • 公司成立:3年
  • 注冊資本:200萬人民幣
  • 企業(yè)類型:有限責(zé)任公司(自然人獨資)
  • 主營產(chǎn)品:辣椒素試劑盒,黃曲霉毒素檢測卡等
  • 公司地址:惠濟區(qū)大河路街道中原物流港B6-1-06
詢盤

hygiena 牛奶中酪蛋白/β-乳球蛋白過敏原檢測試劑盒相關(guān)廠家報價

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