環(huán)孢素|T0945

價(jià)格	￥410	￥311	￥606
包裝	100mg	50mg	200mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-02

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產(chǎn)品詳情

中文名稱(chēng):環(huán)孢素	英文名稱(chēng):Cyclosporin A
CAS:59865-13-3	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.99%	產(chǎn)品類(lèi)別: 抑制劑
貨號(hào): T0945

Product Introduction

Bioactivity

名稱(chēng)	Cyclosporin A
描述	Cyclosporin A is a naturally occurring cyclic polypeptide that is the active metabolite of a fungus. Cyclosporin A is an immunosuppressant that binds to procyclins and inhibits calcineurin (IC50=7 nM).
細(xì)胞實(shí)驗(yàn)	Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments. Cells (106) are suspended in 1 ml of complete medium in microcentrifuge tubes; 1 μl of ethanol or of the ethanolic solution of Cyclosporin A is added, and the cells are incubated at 37°C for 1 hr. Cells are washed twice with 1 ml of PBS on ice and lysed in 50μl of hypotonic buffer containing 50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM dithiothreitol; and 50 μg of phenylmethylsulfonyl fluoride, 50 μg of soybean trypsin inhibitor, 5 μg of leupeptin, and 5 μg of aprotinin per ml. Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30°C and then are centrifuged at 4°C for 10 min at 12,000×g.(Only for Reference)
激酶實(shí)驗(yàn)	Phosphatase Assay: Purified bovine brain calcineurin and calmodulin are purchased. Reaction mixtures with purified enzyme contains 100 nM calcineurin, 100 nM calmodulin, and 5 μM 32P-labeled phosphopeptide, in 60 μl (total volume) of assay buffer containing 20 mM Tris (pH 8), 100 mM NaCI, 6 mM MgCl2, 0.5 mM dithiothreitol, 0.1 mg of bovine serum albumin per ml, and either 0.1 mM CaCl2 or 5 mM EGTA. Reaction mixtures with cell lysates contains 20 μl of undiluted lysate, 5 μM 32P-labeled phosphopeptide, and 40 μl of assay buffer. Where indicated, reaction mixtures contains 50 μM peptide 412 or 413 and/or 500 nM okadaic acid, a specific inhibitor of phosphatases 1 and 2A; 500 nM okadaic acid is sufficient for inhibition of Ca2+-independent phosphatases, whereas higher concentrations partially inhibit Ca2+-dependent activity as well. After 15 min at 30°C, reactions are terminated by the addition of 0.5 ml of 100 mM potassium phosphate buffer (pH 7.0) containing 5% trichloroacetic acid. Free inorganic phosphate is isolated by Dowex cation-exchange chromatography and quantitated by scintillation counting as described.
體外活性	方法：膠質(zhì)瘤細(xì)胞 C6 用 Cyclosporin A (10 μM) 在缺氧條件下處理 4 h，使用 Western Blot 方法檢測(cè)靶點(diǎn)蛋白表達(dá)水平。結(jié)果：4 h 缺氧誘導(dǎo)了內(nèi)源性 HIF-1α 蛋白的大量積累。Cyclosporin A 阻止了缺氧誘導(dǎo)的 HIF-1α 的大部分穩(wěn)定。[1] 方法：人結(jié)腸癌細(xì)胞 CACO-2 用 Cyclosporin A (2 μM) 處理 24-72 h，使用 Flow Cytometry 檢測(cè)細(xì)胞周期情況。結(jié)果：在用 Cyclosporin A 處理后的 G0/G1 階段檢測(cè)到細(xì)胞的積聚。[2]
體內(nèi)活性	方法：為檢測(cè)體內(nèi)對(duì)肌肉疾病的活性，將 Cyclosporin A (5 mg/kg in olive oil) 腹腔注射給肌病 Col6a1-/- 小鼠，每天兩次，持續(xù)十天。結(jié)果：Cyclosporin A 影響 Col6a1-/- 小鼠的衛(wèi)星細(xì)胞活性并觸發(fā)再生纖維的形成。[3] 方法：為檢測(cè)對(duì)缺血再灌注損傷 (IRI) 的作用，將 Cyclosporin A (3 mg/kg，在缺血前 1 h 或 10 min；10 mg/kg，在缺血前 10 min) 腹腔注射給缺血再灌注的 C57BL/6J 小鼠。結(jié)果：Cyclosporin A 10 mg/kg-10 min 和 Cyclosporin A 3 mg/kg-1 h 組的死亡率和腎功能顯著高于 Cyclosporin A 3 mg/kg-10 min 組。[4]
存儲(chǔ)條件	keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 60 mg/mL (49.89 mM) Ethanol : 60.1 mg/mL (50 mM) 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 12.03 mg/mL (10 mM), Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
關(guān)鍵字	Phosphatase \| CsA \| Ciclosporin A \| Complement System \| Antibiotic \| Inhibitor \| inhibit \| Cyclosporin A
相關(guān)產(chǎn)品	Cefaclor monohydrate \| Stearic acid \| Tartaric acid disodium dihydrate \| G-418 disulfate \| Doxycycline \| Neomycin sulfate \| Metronidazole \| EDTA copper(II) disodium salt \| Ampicillin sodium \| 3-Phenoxybenzaldehyde \| Sulfamethoxazole sodium \| Kanamycin sulfate
相關(guān)庫(kù)	經(jīng)典已知活性庫(kù) \| 已知活性化合物庫(kù) \| 環(huán)肽庫(kù) \| 天然產(chǎn)物庫(kù) \| FDA 上市藥物庫(kù) \| 藥物功能重定位化合物庫(kù) \| 高通量篩選天然產(chǎn)物庫(kù)

關(guān)鍵字：環(huán)孢素|||環(huán)孢菌素|||Cyclosporine A|||Cyclosporine|||Ciclosporin|TargetMol

公司簡(jiǎn)介

上海陶術(shù)生物科技有限公司為美國(guó)Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國(guó)波士頓、德國(guó)慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專(zhuān)業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實(shí)體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷(xiāo)售到個(gè)性化定制合成；從對(duì)明確靶點(diǎn)的分子篩選到對(duì)明確分子的多靶點(diǎn)篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿(mǎn)足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過(guò)在中國(guó)市場(chǎng)五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過(guò)五百家學(xué)校和各類(lèi)企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊(cè)資本	566.2651萬(wàn)人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營(yíng)行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營(yíng)模式	貿(mào)易,試劑,定制,服務(wù)

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公司成立：12年
注冊(cè)資本：566.2651萬(wàn)人民幣
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公司地址：上海市閘北區(qū)江場(chǎng)三路28號(hào)4樓

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