Human CD27 Ligand / CD70 Protein, His,Flag Tag, active trimer (MALS verified)

分子別名（Synonym）

CD70,CD27LG,TNFSF7,TNFSF7G,CD27L

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CD27 Ligand Protein, His,Flag Tag (CDL-H52Da) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1 ) trimer Design.

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 49.3 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

分化簇70（CD70）也稱為CD27配體（CD27L/CD27LG）、TNFSF7、TNFSF7G，是屬于TNF超家族（TNFSF）的II型跨膜糖蛋白，是活化的T和B淋巴細(xì)胞以及成熟樹突狀細(xì)胞上發(fā)現(xiàn)的表面抗原。CD70與其受體CD27的結(jié)合誘導(dǎo)了T細(xì)胞的啟動(dòng)、效應(yīng)器功能、分化和記憶形成，因此參與了包括T細(xì)胞活化、共刺激T細(xì)胞增殖以及細(xì)胞溶解性T細(xì)胞生成在內(nèi)的生物過程。T細(xì)胞上的CD70提供T細(xì)胞增殖、克隆擴(kuò)增和促進(jìn)效應(yīng)T細(xì)胞形成所需的共刺激信號(hào)。小鼠B細(xì)胞上的CD70已被證明可以抑制活化的B細(xì)胞向漿細(xì)胞的終末分化，并增強(qiáng)對(duì)記憶B細(xì)胞反應(yīng)的承諾。CD70誘導(dǎo)NK細(xì)胞增殖和IFNγ產(chǎn)生。