DN14-CTAB植物基因組DNA快速提取試劑盒
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 規(guī)格 | 報(bào)價(jià) | 特價(jià) |
| DN1402 | CTAB植物基因組DNA快速提取試劑盒 | 100次 | 640 | 448 |
產(chǎn)品介紹：
改進(jìn)的經(jīng)典CTAB植物DNA抽提液內(nèi)（添加多種針對(duì)植物特點(diǎn)的多糖、多酚去除成份）迅速裂解細(xì)胞和滅活細(xì)胞內(nèi)核酸酶，氯仿抽提后通過(guò)離心**多糖、多酚和蛋白質(zhì)（根據(jù)需要，上清中還加入異丙醇離心沉淀基因組DNA，進(jìn)一步去除其它各種雜質(zhì)），然后基因組DNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜，再通過(guò)一系列快速的漂洗－離心的步驟， 進(jìn)一步將多糖、多酚和細(xì)胞代謝物、蛋白等雜質(zhì)去除，**低鹽的洗脫緩沖液將純凈基因組DNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點(diǎn)：
1.離心吸附柱內(nèi)硅基質(zhì)膜全部采用進(jìn)口特制吸附膜，柱與柱之間吸附量差異極小，可重復(fù)性好?？朔藝?guó)產(chǎn)試劑盒膜質(zhì)量不穩(wěn)定的弊端。
2.不需要使用有毒的苯酚等試劑，也不需要乙醇沉淀等步驟。
3.快速，簡(jiǎn)捷，單個(gè)樣品操作一般可在1小時(shí)內(nèi)完成。
4.數(shù)種去多糖、多酚成份和多次柱漂洗確保高純度，OD260/OD280典型的比值達(dá)1.7～1.9，長(zhǎng)度可達(dá)30 kb -50kb，可直接用于PCR，Southern-blot和各種酶切反應(yīng)。
使用該產(chǎn)品發(fā)表的部分文章：
1.Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, DOI 10.1007/s00299-013-1413-6
2.Taxonomy and phylogeny of Ceriporia (Polyporales, Basidiomycota) with an emphasis of Chinese collections. Mycol Progress 2014, 13: 81–93
3.Phlebiporia bubalina gen. et. sp. nov. (Meruliaceae, Polyporales) from southwest China with a preliminary
4.Down-Regulated miR-30a in Clear Cell Renal Cell Carcinoma Correlated with Tumor Hematogenous Metastasis by Targeting Angiogenesis-Specific DLL4. miR-30a Targets DLL4 in RCC Metastasis. June 2013 | Volume 8 | Issue 6 | e67294
5.A preliminary report on decay and canker of Acacia richii caused by Inonotus rickii in China. For. Path. 44 (2014) 82–84 . doi: 10.1111/efp.12088. 2014 Blackwell Verlag GmbH.
6.Hispidaedalea gen. nov. and Griseoporia taiwanense sp. nov. (Gloeophyllales, Basidiomycota) based on morphological and molecular characters. ORIGINAL ARTICLE. Mycol Progress. DOI 10.1007/s11557-014-0966-2.
7.Two new species of the Heterobasidion insulare complex based on morphology and molecular data. Mycoscience 2014, 55(4): 289–298
8.Three homologous genes encoding functional D8-sphingolipid desaturase in Populus tomentosa. Genes Genom 2014, 36: 293–301
9.Isolation, Characterization, and Structure Analysis of a Vacuolar Processing Enzyme Gene (MhVPEγ) from Malus hupehensis (Pamp) Rehd. Appl Biochem Biotechnol 2014, 73: 579–5954
10.Morphological characters and molecular data reveal a new species of Fomitopsis (Polyporales) from southern China. Mycoscience 2014, http://dx.doi.org/10.1016/j.myc.2014.05.0044
11.Morphology and molecular phylogeny for two new species of Fomitopsis (Basidiomycota) from South China. Mycol Progress 2014, 13: 905–914
12.Coltriciella globosa and C. pseudodependens spp. nov. (Hymenochaetales) from southern China based on morphological and molecular characters. Mycoscience 2014, http://dx.doi.org/10.1016/j.myc.2014.06.001
13.Contribution of arbuscular mycorrhizal fungi of sedges to soil aggregation along an altitudinal alpine grassland gradient on the Tibetan Plateau. Environmental Microbiology 2015, (17)8:2841–2857
14.Stability Assay of Three DNA Extraction Methods for Detection of Strawberry Vein Banding Virus. Acta Agriculturae Boreali-Sinica 2015, 30(3):200-204
關(guān)鍵字： CTAB植物基因組;DNA快速提取試劑盒;
杭州昊鑫生物科技股份有限公司成立于2009年，產(chǎn)品和服務(wù)涵蓋生命科學(xué)研究技術(shù)的諸多方面，提供覆蓋分子生物學(xué)、細(xì)胞生物學(xué)、植物學(xué)、生物化學(xué)、蛋白組學(xué)、免疫學(xué)等領(lǐng)域的實(shí)驗(yàn)產(chǎn)品以及生物技術(shù)服務(wù)等。目前一級(jí)代理品牌有：MCE（Medchemexpress）、Biochannel、AATbio、invivogen、Abnova、Atlas、Origene、Biovision、云克?。–loud-clone）、艾德萊(Aidlab)、Cayman、Jackson、Epigentek、Prospec、Sciencell、icellbioscience（賽百慷）、hkABCbio、chondrex、Mybiosource、Abbexa、Innovrsrch、HPI、Hitobiotec、Greerlabs，Ostex，4ADI，LDN等。