Human CD47-coupled Magnetic Beads
背景（Background）
The Human CD47-coupled Magnetic Beads is produced by coupling biotinylated CD47 protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-CD47 antibody from your sample with high specificity.
表達區(qū)間及表達系統(tǒng)（Source）
Human CD47 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139(Accession # Q08722).
應用說明（Application）
This product is intended for immunocapture, biopanning and flow cytometry.
The Isotype control (Cat. No. SMB-B01) is sold separately.
重構方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存儲（Storage）
Upon receipt, please store the beads at -20°C for 1 year in lyophilized state.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Antibody Purification:
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制劑（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
關鍵字： CD47磁珠;CD47 Beads;Magnetic Beads;ACRO;百普賽斯;
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