Human ANGPT2-coupled Magnetic Beads
背景(Background)
The Human ANGPT2-coupled Magnetic Beads is produced by coupling biotinylated human angiopoietin-2/ANGPT2 protein to streptavidin-conjugated magnetic beads. The pre-coupled beads are ready to use for capturing anti-ANGPT2 antibody or TIE2 protein from your sample with high specificity. The uniform size and large surface-to-volume ratio of the beads ensure highly efficent immunocapture in a simple, fast and convenient workflow with high reproducibilty of data.
表達區(qū)間及表達系統(tǒng)(Source)
Human ANGPT2 Protein is expressed from human 293 cells (HEK293). It contains AA Lys 275 - Phe 496(Accession # O15123-1).
應(yīng)用說明(Application)
This product is intended for immunocapture, biopanning and flow cytometry. This is a non-sterile product.
The Isotype control (Cat. No. SMB-B01) is sold separately.
重構(gòu)方法(Reconstitution)
See Certificate of Analysis (CoA) for detailed instruction.
存儲(Storage)
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理(Assay Principles)
Antibody Purification:
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制劑(Formulation)
Please contact us for detailed information.
Contact us for customized product form or formulation.
關(guān)鍵字: ANGPT2磁珠;ANGPT2 Beads;Magnetic Beads;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務(wù)遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。