LIGHT : LTBR [Biotinylated] Inhibitor Screening ELISA Kit
背景（Background）
Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.
原理（Assay Principles）
This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new LIGHT pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human LTBR to immobilized human LIGHT in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated LTBR protein, a human LIGHT protein, an anti-LIGHT neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human LIGHT.
b) Add your molecule of interest to the tests.
c) Add human LTBR-Biotin to bind the coated LIGHT.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
e) Finally, the half maximal inhibitory concentration (IC50) of your compound to LIGHT:LTBR binding will be determined by comparing OD readings among different experimental groups.
應用說明（Application）
This kit is developed for screening for inhibitors of human LIGHT binding to human LTBR.
It is for research use only.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存儲（Storage）
Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
關鍵字： LIGHT ELISA Kit;LIGHT試劑盒;LIGHT ELISA試劑盒;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領域提供關鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。