PNGase F產品信息
別稱：PNGase F,PNGase F,PNGF
物種：Elizabethkingia miricola
屬性：Protein
標記：Unconjugated
產品描述（Product Details）
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
應用說明（Application）
Leaves N-glycan core oligosaccharides intact and suitable for further analysis.
Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination.
單位定義（Unit Definition）
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 μl.
純度（Purity）
>90% as determined by SDS-PAGE.
酶活（Enzyme Activity）
>500 U/μL
內毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
注意事項（Notes）
To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.
PNGase F will not cleave N-linked glycans containing core α1-3 Fucose.
制劑（Formulation）
Supplied as 0.2 μm filtered solution in 20 Mm Tris, 50 mM NaCl, 5 mM EDTA, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
運輸（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存儲（Storage）
This product is stable after storage at:
The product MUST be stored at -20°C or lower upon receipt.
-20°C for 12 months under sterile conditions.
PNGase F分子背景
PNGase F（肽 N- 糖苷酶 F）來源于 Elizabethkingia miricola，是一種高效的酰胺水解酶，可以裂解由天冬酰胺接的高甘露糖、雜合和復雜的寡糖糖蛋白。PNGase F 的切割位點為糖蛋白內側 N- 乙酰葡萄糖胺（GlcNAc）和天冬酰氨殘基之間的酰胺鍵，同時將酶解后蛋白上的天冬氨酰轉化為天冬氨酸。
關鍵字： PNGase F;PNGaseF酶;PNGase F蛋白;ACRO;百普賽斯;
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