Vitronectin產(chǎn)品信息

英文名稱：Vitronectin

中文名稱：玻連蛋白

靶點別稱：VTN,V75,VN,Vitronectin,S-protein,Serum-spreading factor

物種：Human

標(biāo)簽：Tag Free

屬性：Protein

標(biāo)記：Unconjugated

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Vitronectin Protein, Tag Free (VIN-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Leu 478 (Accession # P04004 ).

Predicted N-terminus: Asp 20

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 52.3 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

Vitronectin分子背景

Vitronectin也被稱為S蛋白、VN、VTN、V75。Vitronectin是一種多功能糖蛋白，參與凝血、抑制膜攻擊復(fù)合物（MAC）的形成、細(xì)胞粘附和遷移、傷口愈合和組織重塑。玻連蛋白的主要細(xì)胞來源是肝細(xì)胞。特異性抗血清或基因缺失阻斷Hic（肺炎球菌表面蛋白C（PspC）家族的成員）分別顯著降低了肺炎球菌與可溶性和固定化玻連蛋白以及因子H的結(jié)合。此外，Vitronectin與糖胺聚糖和蛋白多糖相互作用。被整合素家族的某些成員識別，并作為細(xì)胞與底物的粘附分子。末端溶細(xì)胞補(bǔ)體途徑膜損傷作用的抑制劑。