中文名:磷酸二酯酶I 來(lái)源于東部菱背響尾蛇毒液
英文名:Phosphodiesterase I from Crotalus adamanteus venom
中文別名:磷酸二酯酶 | 5'-核酸外切酶 | 寡核苷酸5'-核苷酸水解酶
純度:≥20 units/mg dry weight
貨號(hào):P128636
包裝:100un
Cas號(hào):9025-82-5
存儲(chǔ)溫度:-20°C儲(chǔ)存
產(chǎn)品介紹:
Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+.Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP.
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