產(chǎn)品屬性：
產(chǎn)品名稱 | 規(guī)格 | CAS號 | 型號 |
CA-5f | 10mM*1mLinDMSO 5mg 10mg 25mg 50mg 100mg | 1370032-19-1 | EY-Y0165562 |
Cas No.1370032-19-1
別名 N/A
化學(xué)名 N/A
分子式 C24H24N2O3
分子量 388.46
溶解度 DMSO : 77.5 mg/mL (199.51 mM);H2O : < 0.1 mg/mL (insoluble)
儲存條件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
產(chǎn)品描述：
CA-5f (0-40 μM, 6 hour) concentration- and time-dependently elevates the level of LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs[1].CA-5f (20 μM, 6 hours) inhibits the degradation of autophagosomes when treated alone or in combination Bafilomycin A1 (100 nM) or Chloroquine (30 μM) in A549 cells and HUVECs[1].CA-5f (20 μM) neither impairs the hydrolytic function nor the quantity of lysosomes[1].CA-5f (20 μM, 96 hours) inhibits the growth of A549 cells, and less cytotoxic to normal HUVECs[1].|| Cell Viability Assay[1]||Cell Line:|A549, HUVECs|Concentration:|20 μM|Incubation Time:|96 hours|Result:|Exhibited more cytotoxicity against A549 cells compared with normal HUVECs.|| Western Blot Analysis[1]||Cell Line:|A549 cells and HUVECs|Concentration:|0-40 μM|Incubation Time:|6 hours|Result:|Elevated LC3B-II (a marker to monitor autophagy) and SQSTM1 protein levels in a concentration- and time-dependent manner.
CA-5f (40 mg/kg, i.p., every 2 days for up to 30 days) is well tolerated, and potently inhibits the growth of tumor in nude mice bearing A549 lung cancer cells[1]. CA-5f (40 mg/kg, i.p.) suppresses autophagic flux and induces apoptosis in nude mice bearing A549 lung cancer cells[1].|| Animal Model:|Nude mice bearing A549 lung cancer cells[1]|Dosage:|40 mg/kg|Administration:|Injected via caudal vein, every 2 days for up to 30 days|Result:|Significantly suppressed tumor volume and weight in mice, increased the number of apoptotic cells in mice.
[1]. Zhang L, et al. Identification of compound CA-5f as a novel late-stage autophagy inhibitor with potent anti-tumor effect against non-small cell lung cancer. Autophagy. 2019 Mar;15(3):391-406.
關(guān)鍵字： 1370032-19-1;C24H24N2O3;CA-5f;
上海一研生物科技有限公司Shanghai yiyan bio-technology Co. Ltd.主要從事免疫學(xué)、分子生物學(xué)和常規(guī)生化試劑等為一體的科研產(chǎn)品銷售企業(yè)，公司自成立以來，秉承""全心全意服務(wù)于科研工作者""的企業(yè)理念，立足生物科技領(lǐng)域，運(yùn)用生物技術(shù)和科研試劑，發(fā)展現(xiàn)代生物科技，為各類大中小醫(yī)院及其它醫(yī)療機(jī)構(gòu)、高等院校、科研院所、企事業(yè)單位提供優(yōu)質(zhì)的產(chǎn)品，服務(wù)生物科技領(lǐng)域的科學(xué)研究人員。
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