1370032-19-1
1370032-19-1 結(jié)構(gòu)式
基本信息
CA-5F
CA 5F
CA5F
1370032-19-1
-5-(1H-indol-3-ylmethylene)-1-methyl-, (3E,5E)-
4-Piperidinone, 3-[(3,4-dimethoxyphenyl)methylene]-5-(1H-indol-3-ylmethylene)-1-methyl-, (3E,5E)-
物理化學(xué)性質(zhì)
常見問題列表
Macroautophagy/autophagy
CA-5f (0-40 μM, 6 hour) concentration- and time-dependently elevates the level of LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs.
CA-5f (20 μM, 6 hours) inhibits the degradation of autophagosomes when treated alone or in combination Bafilomycin A1 (100 nM) or Chloroquine (30 μM) in A549 cells and HUVECs.
CA-5f (20 μM) neither impairs the hydrolytic function nor the quantity of lysosomes.
CA-5f (20 μM, 96 hours) inhibits the growth of A549 cells, and less cytotoxic to normal HUVECs.
Cell Viability Assay
| Cell Line: | A549, HUVECs |
| Concentration: | 20 μM |
| Incubation Time: | 96 hours |
| Result: | Exhibited more cytotoxicity against A549 cells compared with normal HUVECs. |
Western Blot Analysis
| Cell Line: | A549 cells and HUVECs |
| Concentration: | 0-40 μM |
| Incubation Time: | 6 hours |
| Result: | Elevated LC3B-II (a marker to monitor autophagy) and SQSTM1 protein levels in a concentration- and time-dependent manner. |
CA-5f (40 mg/kg, i.p., every 2 days for up to 30 days) is well tolerated, and potently inhibits the growth of tumor in nude mice bearing A549 lung cancer cells.
CA-5f (40 mg/kg, i.p.) suppresses autophagic flux and induces apoptosis in nude mice bearing A549 lung cancer cells.
| Animal Model: | Nude mice bearing A549 lung cancer cells |
| Dosage: | 40 mg/kg |
| Administration: | Injected via caudal vein, every 2 days for up to 30 days |
| Result: | Significantly suppressed tumor volume and weight in mice, increased the number of apoptotic cells in mice. |