中文名稱

尿液無細胞循環(huán)RNA純化中提試劑盒（提取試劑盒）

英文名字

Urine Cell-Free Circulating RNA Purification Midi Kit

供應(yīng)商

Norgen Biotek

產(chǎn)品貨號

NGB-57000

背景資料

Recent evidence indicates that cell-free circulating RNA (cf-RNA) including exosomal RNA in urine contains valuable information for the discovery of biomarkers that can help for the early detection of certain cancer types and for monitoring the disease status as well as for the detection of any infectious pathogens. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acid for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum but with a lower concentration; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood)

產(chǎn)品描述

This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples from volumes ranging from 2 mL to 10 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. For a schematic workflow of the protocol click here. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

產(chǎn)品特點

Isolate all sizes of circulating and exosomal RNA, including microRNA; Versatile urine input ranging from 2 mL to 10 mL; No phenol extractions; No carrier RNA Bind and elute all RNA irrespective of size or GC content, without bias; Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 50 μL to 100 μL; Purify high-quality RNA in 40 - 45 minutes.

保存建議

溶液密封室溫(15-25°C)保存，未開封至少保存2年。具體保存情況收到產(chǎn)品后請按說明書存放。

其他

Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術(shù)公司，基于專利的碳化硅膜純化技術(shù)和無苯酚專利配方，生產(chǎn)高品質(zhì)的核酸和蛋白質(zhì)純化及富集產(chǎn)品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發(fā)的科技創(chuàng)新獎，是一家致力于樣品制備并獲得ISO 9001（產(chǎn)品質(zhì)量）、ISO 13485（產(chǎn)品可用于商業(yè)化的醫(yī)療設(shè)備，包括用于體外診斷領(lǐng)域）和ISO 15189（可用于臨床檢測機分子診斷領(lǐng)域的研發(fā)能力）認證的生物科技公司。 目前該公司的產(chǎn)品多達700余種，提供一流的樣品制備試劑盒，應(yīng)用于基因組和蛋白組等不同的領(lǐng)域。其產(chǎn)品主要覆蓋包括各種標本類型的DNA，RNA和蛋白質(zhì)的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。