中文名稱

尿液無細胞循環(huán)DNA純化大提試劑盒（提取試劑盒）

英文名字

Urine Cell-Free Circulating DNA Purification Maxi Kit

供應(yīng)商

Norgen Biotek

產(chǎn)品貨號

NGB-56800

背景資料

Recent evidence indicates that cell-free circulating DNA (cf-DNA) contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood). Urine cell-free circulating DNA (cf-DNA) has been utilized for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases, as well as for investigating fetal DNA that is normally present in the maternal blood. This cf-DNA is usually present as short fragments, generally between 150 and 250 bp or its duplicates, and is derived either from the apoptotic process, necrotic process or from the fetus.

產(chǎn)品描述

This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA as well as viral DNA from fresh, preserved or frozen urine samples from volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. For a schematic workflow of the protocol click here. The purified urine DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, Microarrays and NGS.

產(chǎn)品特點

Isolate all sizes of circulating DNA from fresh, preserved or frozen urine samples; Isolate viral DNA; Versatile urine input volumes (10 mL - 30 mL); Concentrate circulating DNA into a flexible elution volume ranging from (50 μL - 100 μL); Isolate inhibitor-free cell-free circulating DNA; Purify high-quality DNA in 40-45 minutes; Compatible with Norgen’s Urine Preservative and other commercially available urine preservatives.

保存建議

溶液密封室溫(15-25°C)保存，未開封至少保存2年。具體保存情況收到產(chǎn)品后請按說明書存放。

其他

Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術(shù)公司，基于專利的碳化硅膜純化技術(shù)和無苯酚專利配方，生產(chǎn)高品質(zhì)的核酸和蛋白質(zhì)純化及富集產(chǎn)品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發(fā)的科技創(chuàng)新獎，是一家致力于樣品制備并獲得ISO 9001（產(chǎn)品質(zhì)量）、ISO 13485（產(chǎn)品可用于商業(yè)化的醫(yī)療設(shè)備，包括用于體外診斷領(lǐng)域）和ISO 15189（可用于臨床檢測機分子診斷領(lǐng)域的研發(fā)能力）認證的生物科技公司。 目前該公司的產(chǎn)品多達700余種，提供一流的樣品制備試劑盒，應(yīng)用于基因組和蛋白組等不同的領(lǐng)域。其產(chǎn)品主要覆蓋包括各種標本類型的DNA，RNA和蛋白質(zhì)的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。