硫酸肝素蛋白聚糖(HSPG)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Glu19~Glu144
Tags: N-terminal His Tag
Subcellular Location: Membrane
Purity: > 95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% SKL, 5% Trehalose and Proclin300.
Original Concentration: 200μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 4.7
Predicted Molecular Mass: 15.2kDa
Accurate Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved
to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not
vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined
by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no
obvious degradation and precipitation were observed. The loss rate is less than 5% within the
expiration date under appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure . Gene Sequencing (extract)
Figure. SDS-PAGE
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was
used in clinical diagnostic or any other procedures.
特別提示：本公司的所有產品僅可用于科研實驗，嚴禁用于臨床醫(yī)療及其他非科研用途！
關鍵字： SDC2;HSPG1;SYND2;硫酸肝素蛋白聚糖(HSPG)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
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