多糖裂解酶（Polysaccharide Lyase）是一類主要作用于陰離子多糖,通過(guò)β消除機(jī)制降解糖苷鍵形成在232nm波長(zhǎng)有吸收的C4-C5雙鍵的不飽和寡糖。作為一類生物技術(shù)工具常用于多糖的純度和結(jié)構(gòu)分析，以及寡糖的制備。肝素酶可選擇地剪切硫酸化肝素聚糖中葡萄糖胺和糖醛酸之間α(1-4)糖苷鍵,三種不同的肝素酶(酶Ⅰ、酶Ⅱ、酶Ⅲ)具有非常不同的專屬性。
上?；菡\(chéng)生物提供三種肝素酶(heparinase)，分別是Recombinant Heparinase I,Heparinase II和Heparinase III，咨詢訂購(gòu)熱線：021-60498804
Appearance:Lyophilized powdePurity：≥ 95%Specific Activity ：≥ 3.4 IU/mg proteinStabilizer：None or BSAProtein concentration (BCA assay):1.0 mg/mlActivity (using heparin as substrate): 3.8 IU/mgAssay Buffer:100 mM MOPS, 5 mM CaCl 2 , pH 7.0
將200μl肝素溶液（在測(cè)定緩沖液中為2.5 mg/ml）加入含有50μl重組蛋白的孔中肝素酶II（在測(cè)定緩沖液中新制備的5μg），并在動(dòng)力學(xué)中監(jiān)測(cè)232nm處的吸光度在M5 Microplate Reader
用線性回歸分析對(duì)數(shù)據(jù)進(jìn)行擬合，以獲得對(duì)計(jì)算比活度：
HA11785 肝素酶I Recombinant Heparinase I
0.05IU 1580元
0.1IU 2850元
Source: E. coli-derived Gln22-Arg384, with an N-terminal 6-His tag
SDS-PAGE: 43.6 kDa, reducing conditions
Appearance: Supplied as a 0.2 μm filtered frozen solution in 20 mM Tris, 150 mM NaCl, 2.5mM CaCl2, pH 7.4.
HA11612 肝素酶II Recombinant Heparinase II
0.1IU 1580元
0.25IU 3570元
Protein concentration (BCA assay): 0.4 mg/ml
Activity (using heparin as substrate): 2.7 IU/mg
200 μl of heparin solution (2.5 mg/ml in assay buffer) was added into a well containing 50 μl of recombinant
Heparinase II (2.4μg freshly prepared in assay buffer), and the absorbance at 232nm was monitored in a kinetic mode (in 15 s intervals for 2 min) at 37℃ performed in a SpectraMax M5 Microplate Reader (Molecular Devices,USA).
HA11587 肝素酶III Recombinant Heparinase III
0.5IU 1580元
1.0IU 2850元
SDS-PAGE: 75 kDa, reducing conditions
Source: E. coli-derived Gln25-Pro659, with an N-terminal 6-His tag
Appearance: Supplied as a 0.2 μm filtered frozen solution in 50 mM sodium phosphate, pH 7.6.
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