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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
Carfilzomib Impurity B Carfilzomib Impurity B
Hesperidin dihydrochalcone Hesperidin dihydrochalcone 35573-79-6 C28H36O15
Alogliptin Imp.12 Alogliptin Imp.12 2749281-73-8 C25H25N5O3
Ornidazole Impurity 1 Ornidazole Impurity 1 1100750-18-2
Norpterosin B glucoside Norpterosin B glucoside 1226785-88-1 C19H26O7
(2R,4S)-ethyl 5-([1,1'-biphenyl]-4-yl)-4-aMino-2-Methylpentanoate (2R,4S)-ethyl 5-([1,1'-biphenyl]-4-yl)-4-aMino-2-Methylpentanoate 752174-62-2 C20H25NO2
EMpagliflozin  iMpurity 12 EMpagliflozin iMpurity 12
(2S,4R)-Teneligliptin (2S,4R)-Teneligliptin 1404559-15-4 C22H30N6OS
Daclatasvir Impurity Daclatasvir Impurity 1417333-83-5 C40H50N8O6
(3aS,12bS)-2-Methyl-2,3,3a,12b-tetrahydro-1H-dibenzo[2,3:6,7]oxepino[4,5-c]pyrrole (3aS,12bS)-2-Methyl-2,3,3a,12b-tetrahydro-1H-dibenzo[2,3:6,7]oxepino[4,5-c]pyrrole 65576-39-8 C17H17NO
1-[2-[(3,4-diMethylphenyl)thio]phenyl]- Piperazine 1-[2-[(3,4-diMethylphenyl)thio]phenyl]- Piperazine 1293489-74-3 C18H22N2S
2-(7-Methoxy-3,4-dihydronaphthalen-1-yl)acetic acid 2-(7-Methoxy-3,4-dihydronaphthalen-1-yl)acetic acid 27533-69-3 C13H14O3
N-acetyl-N-(2-(7-Methoxynaphthalen-1-yl)ethyl)acetaMide N-acetyl-N-(2-(7-Methoxynaphthalen-1-yl)ethyl)acetaMide 1379005-34-1 C17H19NO3
N-acetyl-N-(2-(7-Methoxynaphthalen-1-yl)ethyl)acetaMide N-acetyl-N-(2-(7-Methoxynaphthalen-1-yl)ethyl)acetaMide 1379005-34-1 C17H19NO3
carfilzoMib iMpurity -A carfilzoMib iMpurity -A
(3S,4R)-1-(4-fluorophenyl)-3-((S)-3-(4-fluorophenyl)-3-hydroxypropyl)-4-(4-hydroxyphenyl)azetidin-2-one (3S,4R)-1-(4-fluorophenyl)-3-((S)-3-(4-fluorophenyl)-3-hydroxypropyl)-4-(4-hydroxyphenyl)azetidin-2-one 1593543-00-0 C24H21F2NO3
ethyl N-((2'-(1H-tetrazol-5-yl)-[1,1'-biphenyl]-4-yl)Methyl)-N-pentanoyl-L-valinate ethyl N-((2'-(1H-tetrazol-5-yl)-[1,1'-biphenyl]-4-yl)Methyl)-N-pentanoyl-L-valinate 1111177-30-0 C26H33N5O3
Rosuvastatin EP impurity A Rosuvastatin EP impurity A 1714147-50-8 C25H34FN3NaO7S*
ApovincaMinic Acid Ethyl Ester N-Oxide ApovincaMinic Acid Ethyl Ester N-Oxide 109741-24-4 C22H26N2O3
Doxorubicin Impurity 6 Doxorubicin Impurity 6 69429-21-6 C26H27NO11
Etoricoxib Impurity 11 Etoricoxib Impurity 11 202409-31-2 C17H13ClN2O2S
Levofloxacin Tetrafluoro Impurity 1 Levofloxacin Tetrafluoro Impurity 1 110548-02-2 C15H15F4NO4
Penicillin F Potassium Salt Penicillin F Potassium Salt 97299-13-3 C14H21KN2O4S
Olaparib Impurity 31 Olaparib Impurity 31 458535-34-7 C12H18N2O2
Macitentan impurity A Macitentan impurity A 441798-25-0 C17H16Br2N6O4S
4-(3,4-DICHLOROPHENYL)-3,4-DIHYDRO-2H-NAPHTHALEN-1-ONE OXIME 4-(3,4-DICHLOROPHENYL)-3,4-DIHYDRO-2H-NAPHTHALEN-1-ONE OXIME 152642-35-8 C16H13Cl2NO
Lucas Lucas
Afatinib Impurity M Afatinib Impurity M
Ornidazole  Impurity Ornidazole Impurity 1100750-18-2
4-(3-nitrophenyl)-3-Morpholinone 4-(3-nitrophenyl)-3-Morpholinone 845729-43-3 C10H10N2O4
1,3-bis(2-chloroethyl)benzimidazolin-2-one 1,3-bis(2-chloroethyl)benzimidazolin-2-one 55470-74-1 C11H12Cl2N2O
Nintedanib Impurity A (Intedanib Impurity A ) Nintedanib Impurity A (Intedanib Impurity A )
Tigecycline Impurity 6 Tigecycline Impurity 6
Febuxostat Bromo Impurity Febuxostat Bromo Impurity 144060-40-2 C15H16BrNO3S
Meptazinol Impurity Meptazinol Impurity 1314003-47-8 C23H28N2O4
1'H-2,3'-bipyridinyl-2'-one 1'H-2,3'-bipyridinyl-2'-one 101002-01-1 C10H8N2O
Brexpiprazole Impurity D Brexpiprazole Impurity D
Brivaracetam Impurity E Brivaracetam Impurity E
Dapoxetine Impurity 8 Dapoxetine Impurity 8
Irbesartan Impurity 6 Irbesartan Impurity 6 C25H28N6O
Abiraterone Impurity 9 Abiraterone Impurity 9 165334-72-5 C24H31NO
Gliclazide Impurity A Gliclazide Impurity A
Medetomidine Impurity 14 Medetomidine Impurity 14 79924-14-4 C13H16N2
Tenofovir Disoproxil Impurity B Tenofovir Disoproxil Impurity B 36817-69-3 C8H11N5O
Ipragliflozin  IMP Ipragliflozin IMP
2-Hexenoic acid, 2-cyano-5-methyl- 2-Hexenoic acid, 2-cyano-5-methyl- 869-02-3 C8H11NO2
CP-690550A CP-690550A 1243290-37-0 C15H21N5O2
(S)-Tomoxetine (S)-Tomoxetine 105314-53-2 C17H21NO
Fexofenadine EP Impurity F Fexofenadine EP Impurity F 185066-33-5 C31H37NO4
(E)-2-(7-methoxy-3,4-dihydronaphthalen-1(2H)-ylidene)ethan-1-amine (E)-2-(7-methoxy-3,4-dihydronaphthalen-1(2H)-ylidene)ethan-1-amine 468104-18-9 C13H17NO
4-(5-(pyridin-4-yl)-1H-1,2,4-triazol-3-yl)picolinamide 4-(5-(pyridin-4-yl)-1H-1,2,4-triazol-3-yl)picolinamide 1992028-94-0 C13H10N6O
Indacaterol Impurity 22 Indacaterol Impurity 22 773895-24-2 C31H34N2O3
10-O-2,2-Dichloroethoxycarbonyl Docetaxe 10-O-2,2-Dichloroethoxycarbonyl Docetaxe 158810-73-2 C46H55Cl2NO16
4R,5S)-2,4-diphenyl-4,5-dihydrooxazole-5-carboxylic acid 4R,5S)-2,4-diphenyl-4,5-dihydrooxazole-5-carboxylic acid 849831-77-2 C16H13NO3
1-(4-AMinophenyl)-5,6-dihydro-3-(4-Morpholinyl)-2(1h)-pyridinone 1-(4-AMinophenyl)-5,6-dihydro-3-(4-Morpholinyl)-2(1h)-pyridinone 1267610-26-3 C15H19N3O2
Erlotinib IMpurity C Erlotinib IMpurity C 1312937-41-9 C15H20N2O5
Cinacalcet IMpurity Cinacalcet IMpurity 1229224-93-4 C22H26F3N
Empagliflozin impurity B Empagliflozin impurity B
Varenicline Impurity 1 Varenicline Impurity 1
Voriconazole Impurity 6 Voriconazole Impurity 6
Caspofungin impurity 1 Caspofungin impurity 1
Cabozantinib impurity D Cabozantinib impurity D
Neratinib Impurity 3 Neratinib Impurity 3
Ranitidine Impurity D Ranitidine Impurity D C13H22N4O3S
Metoprolol IMpurity 5 Metoprolol IMpurity 5 C15H25NO3
Mirabegron Impurity 2 Mirabegron Impurity 2
Pemetrexed Impurity 1 Pemetrexed Impurity 1 1644287-26-2 C20H21N5O8
Plerixafor Impurity B Plerixafor Impurity B
Tolvaptan impurity3 Tolvaptan impurity3
Vonoprazan Impurity 7 Vonoprazan Impurity 7
Cefazolin impurity F Cefazolin impurity F
Apremilast Impurity 3 Apremilast Impurity 3 88687-92-7 C16H10N2O8
Apixaban Impurity 8 Apixaban Impurity 8 1777781-49-3 C24H23N5O4
Ritodrine BP Impurity D Ritodrine BP Impurity D
Rivaroxaban Impurity 19 Rivaroxaban Impurity 19
Topiroxostat Impurity 4 Topiroxostat Impurity 4 1379342-46-7 C8H8N2O3
Ertapenem impurity 1 Ertapenem impurity 1
(R)-2-(3-(diisopropylamino)-1-phenylpropyl)-4-(hydroxymethyl)phenyl methacrylate (R)-2-(3-(diisopropylamino)-1-phenylpropyl)-4-(hydroxymethyl)phenyl methacrylate C26H36NO3
Flomoxef Impurity 3 Flomoxef Impurity 3
Ropivacaine impurity Ropivacaine impurity 1105704-96-8 C12H14N2O3
Agomelatine Impurity 1 Agomelatine Impurity 1 1352139-51-5 C15H19NO2
Diquafosol Impurity 1 Diquafosol Impurity 1 26184-65-6 C18H24N4O17P2
Milnacipran Impurity 3 Milnacipran Impurity 3 1353644-50-4 C15H20ClNO
L-Glutamic acid 1-ethyl ester L-Glutamic acid 1-ethyl ester 52454-78-1 C7H13NO4
Methyl 2-Hydroxy-2-(Thiophen-2-Yl)-2-(Thiophen-3-Yl)Acetate Methyl 2-Hydroxy-2-(Thiophen-2-Yl)-2-(Thiophen-3-Yl)Acetate 28748-67-6 C11H10O3S2
6,7-Dihydro-N-methyl-N-[(3R,4R)-4-methyl-3-piperidinyl]-5H-pyrrolo[2,3-d]pyrimidin-4-amine 6,7-Dihydro-N-methyl-N-[(3R,4R)-4-methyl-3-piperidinyl]-5H-pyrrolo[2,3-d]pyrimidin-4-amine 1812890-23-5 C13H21N5
Sacubitril Impurity 72 Sacubitril Impurity 72 1361408-16-3 C23H27NO4
(1R,2S,3R)-Aprepitant (1R,2S,3R)-Aprepitant 1185502-97-9 C23H21F7N4O3
Clemastine Clemastine 108825-05-4 C21H26ClNO2
4-chloro-7-methoxyquinoline-6-carboxylic acid 4-chloro-7-methoxyquinoline-6-carboxylic acid 771464-30-3 C11H8ClNO3
ethyl (41S,13aR)-13a-ethyl-2,3,41,5,6,13a-hexahydro-1H-indolo[3,2,1-de]pyrido[3,2,1-ij][1,5]naphthyridine-12-carboxylate ethyl (41S,13aR)-13a-ethyl-2,3,41,5,6,13a-hexahydro-1H-indolo[3,2,1-de]pyrido[3,2,1-ij][1,5]naphthyridine-12-carboxylate 85647-43-4 C22H26N2O2
methyl (41R,12R,13aR)-13a-ethyl-12-hydroxy-2,3,41,5,6,12,13,13a-octahydro-1H-indolo[3,2,1-de]pyrido[3,2,1-ij][1,5]naphthyridine-12-carboxylate methyl (41R,12R,13aR)-13a-ethyl-12-hydroxy-2,3,41,5,6,12,13,13a-octahydro-1H-indolo[3,2,1-de]pyrido[3,2,1-ij][1,5]naphthyridine-12-carboxylate 38990-16-8 C21H26N2O3
Dolutegravir Impurity 3 Dolutegravir Impurity 3 1973402-05-9 C20H19F2N3O5
Cistracurium Besylate EP Impurity D Cistracurium Besylate EP Impurity D 1075726-88-3 C31H39NO9S
(R)-1-[3-[4-Amino-3-(4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl]piperidin-1-yl]-3-chloropropan-1-one (R)-1-[3-[4-Amino-3-(4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl]piperidin-1-yl]-3-chloropropan-1-one 1288338-96-4 C25H25ClN6O2
Bendamustine Impurity 8 Bendamustine Impurity 8 1797881-48-1 C14H19Cl2N3O2
FeBuxostat Impurity 32 FeBuxostat Impurity 32 1805770-41-5 C12H12N2O
Edoxaban Impurity 9(1R,2S,4S) Edoxaban Impurity 9(1R,2S,4S) 2024614-15-9 C21H30ClN5O5
Rivaroxaban Impurity 22 Rivaroxaban Impurity 22 1350288-77-5 C19H18ClN3O5S
Rivaroxaban Impurity 27 Rivaroxaban Impurity 27 1369969-44-7 C21H21N3O5
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